Macrophage control of mycobacterial growth induced by picolinic acid is dependent on host cell apoptosis

• Type: Article in International Scientific Journal
• Date: 2000

Authors

Pais, TF (Author) Other; Appelberg, R (Author) ICBAS

Journal

Title: Journal of Immunology

Vol. 164

Pages: 389-397

ISSN: 0022-1767

Publisher: American Association of Immunologists

Indexing

ISI Web of Knowledge - 45 Citations

Scopus - 49 Citations

Pubmed / Medline

Scientific classification

FOS: Medical and Health sciences > Basic medicine

CORDIS: Health sciences > Medical sciences > Medicine > Infections

Other information

Authenticus ID: P-001-1S1

Abstract (EN): The effects of picolinic acid (PA) on the intramacrophagic growth of Mycobacterium avium were studied. PA reduced M. avium growth inside mouse macrophages and led to a complete control of mycobacterial growth when added together with IFN-gamma. The mechanism involved did not require TNF-alpha, NO, or the respiratory burst, and was not dependent on either iron or,zinc withholding, The mycobacteriostatic activity of the macrophages was associated with the induction of morphological changes that culminated in apoptosis at day 4 of treatment. PA alone induced apoptosis in macrophages, and this effect was increased by IFN-gamma treatment. Apoptosis at day 4 of infection was reduced by inhibiting macrophage activation with the prostaglandin 15 deoxyprostaglandin J(2) or by treating the cells with the antioxidant N-acetylcysteine. Mycobacterial growth was partially restored in macrophages treated with PA and IFN-gamma when 15 deoxy-prostaglandin J(2) was added, concomitant with a delay in apoptosis, N-Acetylcysteine or glutathione could also completely revert the mycobacteriostatic effects of PA or PA plus IFN-gamma.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9