Localization and Properties of Cholinesterases in the Common Prawn (Palaemon serratus): a Kinetic-Histochemical Study

• Type: Article in International Scientific Journal
• Date: 2010

Authors

M. F. Frasco (Author) ICBAS; I. Erzen (Author) Other; J. Stojan (Author) Other; L. Guilhermino (Author) ICBAS

Journal

Title: Biological Bulletin

Vol. 218

Pages: 1-5

ISSN: 0006-3185

Publisher: University of Chicago Press

Indexing

ISI Web of Knowledge - 3 Citations

ISI Web of Science

Scopus - 5 Citations

Pubmed / Medline

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-003-986

Abstract (EN): Due to the diversity in biochemical Properties and tissue distribution of cholinesterase (ChE) enzymes, a characterization should be performed before they are used as biomarkers for monitoring pesticide contamination. In this study We investigate the distribution of ChEs (aid their kinetic properties in diverse tissues of the common prawn Palaemon serratus. The results concerning the histochemical localization of ChEs suggest that the highest amount of ChE activity occurs in Prawn eyes, followed by the brain, gills, and digestive tract. Negligible staining was observed in the muscle. We describe the kinetic properties of ChEs in eyes, gills, and hepatopancreas, investigating their substrate preferences with different thiocholine esters and their sensitivity to inhibition with selective inhibitors. The results suggest that the studied enzymes are ChEs and not nonspecific esterases, due to their apparent affinity for choline esters, with a distinct preference for the substrate acetyl-thiocholine, and their high sensitivity to inhibition by eserine sulfate. P. serratus eyes can be considered the best tissue for recovery of ChE enzyme: this tissue is easy to isolate, it expresses high levels of ChE, and the enzyme shows Properties of a vertebrate AChE.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5