Effects of widely used pharmaceuticals and a detergent on oxidative stress biomarkers of the crustacean Artemia parthenogenetica

• Type: Article in International Scientific Journal
• Date: 2006

Authors

Nunes, B (Author) Other; Carvalho, F (Author) FFUP; Guilhermino, L (Author) ICBAS

Journal

Title: Chemosphere

Vol. 62

Pages: 581-594

ISSN: 0045-6535

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 68 Citations

ISI Web of Science

Scopus - 87 Citations

Pubmed / Medline

Scientific classification

FOS: Natural sciences > Earth and related Environmental sciences

CORDIS: Natural sciences > Environmental science > Ecology > Ecotoxicology

Other information

Authenticus ID: P-004-PAV

DOI: 10.1016/j.chemosphere.2005.06.013

Abstract (EN): Pharmaceuticals are continuously dispersed into the environment as a result of human and veterinary use, posing relevant environmental concerns. The present paper reports the acute toxic effects of three therapeutic agents (diazepam, clofibrate and clofibric acid) and a detergent, sodium dodecylsulphate (SDS), to the hypersalinc crustacean Artemia parthenogenetica. This study specially focused on oxidative stress parameters, namely (1) total and selenium-dependent glutathione-peroxidase (GPx), (2) glutathione reductase (GRed), (3) total Superoxide dismutase (SOD), and (4) glutathione-S-traiisferases (GSTs). The effects of tested substances on lipid peroxidation (thiobarbituric acid reactive substances, TBARS), and soluble cholinesterases (ChE) were also investigated. Diazepam caused a significant inhibition of ChE (LOEC = 7.04 mg/l) and total GPx activities. SDS was responsible for a decrease in the activity of both ChE (LOEC = 8.46 mg/l) and GRed (LOEC = 4.08 mg/l). Both fibrates (clofibrate and clofibric acid) were responsible for significant decreases in Se-dependent GPx, with LOEC values of 176.34 and 3.09 mg/l, respectively. Clofibrate also caused a slight increase of TBARS content of A. parthenogenetica homogenates. These results indicate that the exposure to all the tested compounds induced alterations on the Cellular redox status in A. parthenogenetica. In addition, diazepam was shown to have the capability of interfering with A. parthenogenetica neurotransmission, through the inhibition of ChE.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 14