TWIST1 Gene: First Insights in Felis catus

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Baptista CS (Author) ICBAS; Bastos E (Author) Other; Santos S (Author) Other; Gut IG (Author) Other; Guedes-Pinto H (Author) Other; Fátima Gartner (Author) ICBAS; Chaves R (Author) Other

Journal

Title: Current Genomics

Vol. 11 No. 3

Pages: 212-220

ISSN: 1389-2029

Publisher: Bentham Science

Indexing

ISI Web of Knowledge - 3 Citations

ISI Web of Science

Scopus - 4 Citations

Pubmed / Medline

Scientific classification

FOS: Agrarian Sciences > Veterinary science

Other information

Authenticus ID: P-003-6XN

DOI: 10.2174/138920210791110933

Abstract (EN): TWIST1 is thought to be a novel oncogene. Understanding the molecular mechanisms regulating the TWIST1 gene expression profiles in tumor cells may give new insights regarding prognostic factors and novel therapeutic targets in veterinary oncology. In the present study we partially isolated the TWIST1 gene in Felis catus and performed comparative studies. Several primer combinations were used based on the alignments of homologous DNA sequences. After PCR amplification, three bands were obtained, purified and sequenced. Several bioinformatic tools were utilized to carry out the comparative studies. Higher similarity was found between the isolated TWIST1 gene in Felis catus and Homo sapiens (86%) than between Homo sapiens and Rattus norvegicus or Mus musculus (75%). Partial amino acid sequence showed no change in the four species analyzed. This confirmed that coding sequences presented high similarity (similar to 96%) between man and cat. These results give the first insights regarding the TWIST1 gene in cat but further studies are required in order to establish, or not, its role in tumor formation and progression in veterinary oncology.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9