Wound healing activity of the human antimicrobial peptide LL37

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Reinaldo Ramos (Author) Other; Joao Pedro Silva (Author) Other; Ana Cristina Rodrigues (Author) Other; Raquel Costa (Author) Other; Luisa Guardao (Author) Other; Fernando Schmitt (Author) FMUP; Raquel Soares (Author) FMUP; Manuel Vilanova (Author) ICBAS; Lucilia Domingues (Author) Other; Miguel Gama (Author) Other

Journal

Title: Peptides

Vol. 32 No. 1469-1476

Pages: 1469-1476

ISSN: 0196-9781

Publisher: Elsevier

Indexing

ISI Proceedings

ISI Web of Knowledge - 204 Citations

Scopus - 210 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

CORDIS: Health sciences > Medical sciences > Medicine > General pathology

Other information

Authenticus ID: P-002-PWW

DOI: 10.1016/j.peptides.2011.06.005

Abstract (EN): Antimicrobial peptides (AMPs) are part of the innate immune system and are generally defined as cationic, amphipathic peptides, with less than 50 amino acids, including multiple arginine and lysine residues. The human cathelicidin antimicrobial peptide LL37 can be found at different concentrations in many different cells, tissues and body fluids and has a broad spectrum of antimicrobial and immunomodulatory activities. The healing of wound is a complex process that involves different steps: hemostasis, inflammation, remodeling/granulation tissue formation and re-epithelialization. Inflammation and angiogenesis are two fundamental physiological conditions implicated in this process. We have recently developed a new method for the expression and purification of recombinant LL37. In this work, we show that the recombinant peptide P-LL37 with a N-terminus proline preserves its immunophysiological properties in vitro and in vivo. P-LL37 neutralized the activation of macrophages by lipopolysaccharide (LPS). Besides, the peptide induced proliferation, migration and tubule-like structures formation by endothelial cells. Wound healing experiments were performed in dexamethasone-treated mice to study the effect of LL37 on angiogenesis and wound regeneration. The topical application of synthetic and recombinant LL37 increased vascularization and re-epithelialization. Taken together, these results clearly demonstrate that LL37 may have a key role in wound regeneration through vascularization.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8