Tumourigenic effect of Schistosoma haematobium total antigen in mammalian cells

• Type: Article in International Scientific Journal
• Date: 2009

Authors

Botelho, M (Author) Other; Oliveira, P (Author) Other; Gomes, J (Author) Other; Fátima Gärtner (Author) ICBAS; Lopes, C (Author) Other; Correia da Costa, JMC (Author) Other; Machado JC (Author) FMUP

Journal

Title: International Journal of Experimental Pathology

Vol. 90

Pages: 448-453

ISSN: 0959-9673

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 22 Citations

Scopus - 23 Citations

Other information

Authenticus ID: P-003-H2R

DOI: 10.1111/j.1365-2613.2009.00650.x

Abstract (EN): P>Schistosoma haematobium is endemic in several regions of Africa and has been shown to be associated with predominantly squamous cell bladder carcinoma. The mechanisms underlying the association between S. haematobium and bladder squamous cell carcinoma is largely unknown. All the reports so far, demonstrate exclusively an epidemiological evidence linking S. haematobium infection with squamous cell bladder carcinoma. We hypothesized that these parasite antigens might induce tumourigenesis. For this, we used normal mammalian cells of Chinese hamster ovary (CHO) and treated the cells in culture with S. haematobium total antigen (Sh). Our results showed increased proliferation in Sh-treated cells in comparison with the controls. The CHO cells exposed to Sh were inoculated subcutaneously into male nude mice and formed sarcomas (n = 5/5). The cells from the sarcomas expressed vimentin filaments and were negative to cytokeratin. Our results demonstrate for the first time that S. haematobium antigens induce tumour development in nude mice.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6