An assessment of the clonality of the components of canine mixed mammary tumours by mitochondrial DNA analysis

• Type: Article in International Scientific Journal
• Date: 2009

Authors

Angelica C Bertagnolli (Author) Other; Paula Soares (Author) FMUP; Barbara van Asch (Author) Other; Antonio Amorim (Author) FCUP; Luis Cirnes (Author) Other; Valdemar Maximo (Author) FMUP; Geovanni D Cassali (Author) Other

Journal

Title: Veterinary Journal

Vol. 182

Pages: 269-274

ISSN: 1090-0233

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 18 Citations

Scopus - 19 Citations

Scientific classification

FOS: Agrarian Sciences > Veterinary science

Other information

Authenticus ID: P-003-EYM

DOI: 10.1016/j.tvjl.2008.07.005

Abstract (EN): The aim of this study was to investigate if mutations in the mitochondrial DNA (mtDNA) D-loop fragment control region of canine mammary mixed tumours could be used as clonal markers that identified the cell population of origin. Ten benign mixed mammary tumours and nine carcinomas arising from benign mixed tumours were microdissected and DNA from epithelial and mesenchymal tumour cells and from normal mammary tissue was examined for sequence variations in a fragment of the hypervariable control region. Identical sequence variants in both the epithelial and triesenchymal components (as well as in the corresponding normal tissue) were found in 80% of the benign mixed tumours and in 89% of the carcinomas arising from benign mixed tumours suggesting a shared clonal origin. The distinctive sequence alterations identified in the epithelial and mesenchymal components of 15.8%, of all 19 tumours examined, suggests the possibility that a minority of mammary tumours are polyclonal in origin or that early clonal divergence occurs. Increased mutation within the mtDNA D-loop fragment of mixed tumour components was not observed.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6