Exercise training protects the heart from acute pressure overload by decreasing apoptosis and NF-KB activation.

• Type: Summary of Presentation in a National Conference
• Date: 2010

Authors

Moreira-Gonçalves D (Author) Other; Henriques-Coelho T (Author) FMUP; Fonseca H (Author) Other; Gonçalves N (Author) FMUP; Alberto-Duarte J (Author) Other; Leite Moreira AF (Author) FMUP

Conference proceedings National

Title: Revista Portuguesa Cardiologia

Initial page: I-92

XXXI Congresso Português de Cardiologia

Lisboa, 09 a 12 de Abril de 2010

Scientific classification

FOS: Medical and Health sciences > Other medical sciences

CORDIS: Health sciences > Medical sciences > Medicine > Cardiology

Other information

Resumo (PT): Introduction: Cardiac overload is considered an important stimulus for triggering the inital steps to HF. We hypothesized that exercise training (ExT) could positively influence the early cardiac adaptation to overload by preserving myocardial integrity and modulating the triggers of pathways associated with pathological cardiac remodeling. Material and Methods: Male Wistar rats were randomly divided in 3 groups: Exercise Overload (Ex+Ov; n=5; 14 week-ExT and acute pressure overload at the end of the study), Control (CT; n=5; no exercise and no overload) and Control Overload (CT+Ov; n=5; overload in non exercised animals). At the end of the ExT protocol, animals were prepared for hemodynamic instrumentation with tip micromanometer pressure catheters. An acute pressure overload (APO) was induced by constricting the descending aorta (60% LVPmax elevation) for 120 min. Samples from cardiac tissue were prepared for Light (LM) and Transmission Electron Microscopy (TEM) and immunohistochemistry (cleaved caspase-3 and Nf-kB). Results: In comparison with CT group, CT+Ov animals presented intracellular edema and mitochondrial swelling in both ventricles. Regarding hemodynamic function, alterations were limited to LV, which showed diastolic dysfunction. Conversely, in Ex+Ov group, APO resulted in mild signs of cardiac interstitial edema in both ventricles, with no detectable LV or RV dysfunction. Cleaved caspase-3 and Nf-KB was significantly expressed only in cardiomyocytes from LV of CT+Ov group. There was no significant expression of caspase-3 or Nf-kB in cardiomyocytes from RV of both groups. Conclusion: The present study shows that apoptosis and Nf-kB pathways are activated as early as 2 hours after LV APO and that ExT seems to be effective in reducing their activation. We demonstrated that contrarily to sedentary animals, ExT provided functional and ultra-structural protection in both ventricles. These findings suggest that exercise training has an important protective role in the modulation of the early cardiac response to pressure overload.

Language: Portuguese

Type (Professor's evaluation): Scientific

Notes: XXXI Congresso Português de Cardiologia, publicado na Rev. Port. Cardiol. 2010; Vol.29(Sup.I):I-92.