Lymphatic Vessel Density in the Normal-Looking Columnar Epithelium Adjacent to and Distant from Prostatic Intraepithelial Neoplasia and Prostate Cancer Assessed in Whole-Mount Sections

• Type: Article in International Scientific Journal
• Date: 2009

Authors

Schmitt F (Author) FMUP

Journal

Title: Analytical and Quantitative Cytology and Histology

Vol. 31

Pages: 269-275

ISSN: 0884-6812

Publisher: Science Printers and Publishers

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 2 Citations

Scientific classification

FOS: Medical and Health sciences > Other medical sciences

Other information

Authenticus ID: P-003-F4V

Abstract (EN): OBJECTIVE: To evaluate the lymphatic vessel density (LVD) in radical prostatectomy using the lymphatic marker D2-40 and analyze if LVD is variable within the whole-mounting samples of prostatectomy in different areas of carcinoma, high- and low-grade prostatic intraepithelial neoplasia and nodular hyperplasia. STUDY DESIGN: From each of the 4 cases used for this study, we divided the whole-mount slides into 12 regions. Forty-eight regions were examined immunohistochemically. The LVD of D2-40 positive endothelial cells into the radical prostatectomy was estimated by drawing the areas of interest (lymphatic endothelium) dispersed along the prostate zone to facilitate the counting of the positive cells within prostate areas (normal-looking cells, hyperplastic cells, intraepithelial neoplasia and invasive neoplasia). The results were expressed as number of cells per area. RESULTS: LVD counting for nodular hyperplasia of prostate, low-grade prostatic intraepithelial neoplasia, high-grade prosta tic intraepithelial neoplasia and prostate carcinoma was 1.29, 1.20, 1.26 and 1.13, respectively. The statistical analysis (p = 0.265) showed an absence of significant differences of LVD analysis among the lesions. CONCLUSIONS: LVD in prostatic cancer does not appear importantly enhanced around tumoral stroma. The lymphatic vessel distribution was quite similar around malignant and nonmalignant areas within the same prostatectomy samples. (Anal Quant Cytol Histol 2009;31:269-275)

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7