Age-related changes in renal expression of oxidant and antioxidant enzymes and oxidative stress markers in male SHR and WKY rats

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Sónia Simão (Author) Other; Pedro Gomes (Author) Other; Vanda Pinto (Author) Other; Elisabete Silva (Author) Other; Bruno Igreja (Author) Other; Joana Afonso (Author) Other; Maria Paula Serrão (Author) Other; Maria João Jorge Pinho (Author) FMUP; Patrício Soares da Silva (Author) FMUP

Journal

Title: Experimental Gerontology

Vol. 46 No. 7

Pages: 468-474

ISSN: 0531-5565

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 24 Citations

Scopus - 26 Citations

Pubmed / Medline

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-002-QXP

DOI: 10.1016/j.exger.2011.02.003

Abstract (EN): Oxidative stress has been hypothesized to play a role in aging and age-related disorders, such as hypertension. This study compared levels of oxidative stress and renal expression of oxidant and antioxidant enzymes in male normotensive Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHR) at different ages (3 and 12 months). In the renal cortex of 3-month old SHR increases in hydrogen peroxide (H2O2) were accompanied by augmented expression of NADPH oxidase subunit Nox4 and decreased expression of antioxidant enzymes SOD1 and SOD3. A further increase in renal H2O2 production and urinary TBARS was observed in 12-month old WKY and SHR as compared with 3-month old rats. Similarly, expressions of NADPH oxidase subunit p22(Phox), SOD2 and SOD3 were markedly elevated with age in both strains. When compared with age-matched WKY, catalase expression was increased in 3-month old SHR, but unchanged in 12-month old SHR. Body weight increased with aging in both rat strains, but this increase was more pronounced in WKY. In conclusion, renal oxidative stress in 12-month old SHR is an exaggeration of the process already observed in the 3-month old SHR, whereas the occurrence of obesity in 12-month old normotensive rats may partially be responsible for the age-related increase in oxidative stress. (C) 2011 Published by Elsevier Inc.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7