Renal aging in WKY rats: Changes in Na+,K+-ATPase function and oxidative stress

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Elisabete Silva (Author) Other; Vanda Pinto (Author) Other; Sónia Simão (Author) Other; Maria Paula Serrão (Author) Other; Joana Afonso (Author) Other; João Amaral (Author) Other; Maria João Pinho (Author) FMUP; Pedro Gomes (Author) Other; Patrício Soares da Silva (Author) FMUP

Journal

Title: Experimental Gerontology

Vol. 45 No. 12

Pages: 977-983

ISSN: 0531-5565

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 7 Citations

Scopus - 7 Citations

Pubmed / Medline

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-003-0G6

DOI: 10.1016/j.exger.2010.09.012

Abstract (EN): It has been suggested that alterations in Na+,K+-ATPase mediate the development of several aging-related pathologies, such as hypertension and diabetes. Thus, we evaluated Na+,K+-ATPase function and H2O2 production in the renal cortex and medulla of Wistar Kyoto (WKY) rats at 13, 52 and 91 weeks of age. Creatinine clearance, proteinuria, urinary excretion of Na+ and K+ and fractional excretion of Na+ were also determined. The results show that at 91 weeks old WKY rats had increased creatinine clearance and did not have proteinuria. Despite aging having had no effect on urinary Na+ excretion, urinary K+ excretion was increased and fractional Na+ excretion was decreased with age. In renal proximal tubules and isolated renal cortical cells, 91 week old rats had decreased Na+,K+-ATPase activity when compared to 13 and 52 week old rats. In renal medulla, 91 week old rats had increased Na+,K+-ATPase activity, paralleled by an increase in protein expression of alpha(1)-subunit of Na+,K+-ATPase. In addition, renal H2O2 production increased with age and at 91 weeks of age renal medulla H2O2 production was significantly higher than renal cortex production. The present work demonstrates that although at 91 weeks of age WKY rats were able to maintain Na+ homeostasis, aging was accompanied by alterations in renal Na+,K+-ATPase function. The observed increase in oxidative stress may account, in part, for the observed changes. Possibly, altered Na+,K+-ATPase renal function may precede the development of age-related pathologies and loss of renal function.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7