An improved protocol for bacteria identification by MALDI-TOF MS directly from positive blood cultures

• Type: Another Publication in an International Scientific Journal
• Date: 2024

Authors

Cruz, S (Author) Other; Abreu, D (Author) Other; Gomes, R (Author) Other; Martins Oliveira, I (Author) Other; Silva Dias, A (Author) Other; Perez Viso, B (Author) Other; Cantón, R (Author) Other; pina-vaz, c (Author) FMUP

Journal

Title: European Journal of Clinical Microbiology and Infectious Diseases

ISSN: 0934-9723

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 1 Citation

Scopus - 1 Citation

Other information

Authenticus ID: P-00Z-KJQ

DOI: 10.1007/s10096-023-04725-3

Abstract (EN): FASTinov (R) developed a rapid antimicrobial susceptibility test that includes the purification of a bacterial suspension directly from positive blood cultures (BC). In order to streamline laboratory workflow, the use of the bacterial suspension obtained through FASTinov (R) sample prep was tested for identification (ID) by matrix absorption laser deionization-time of flight mass spectrometry (MALDI-TOF MS) (Bruker) in 364 positive BC, and its accuracy assessed comparing with the MALDI-TOF MS ID of the next-day subcultured colonies. FASTinov sample prep was highly reliable for rapid ID directly from BC with proportion of agreement of 94.9% for Gram-positive and 96.3% for Gram-negative bacteria.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6