Dynamic Culture of Osteogenic Cells in Biomimetically Coated Poly(Caprolactone) Nanofibre Mesh Constructs

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Araujo, JV (Author) Other; Cunha Reis, C (Author) Other; Rada, T (Author) Other; da Silva, MA (Author) Other; Gomes, ME (Author) Other; Yang, Y (Author) Other; Ashammakhi, N (Author) Other; Rui Reis (Author) Other; El Haj, AJ (Author) Other; Neves, NM (Author) Other

Journal

Title: Tissue Engineering - Part A

Vol. 16

Pages: 557-563

ISSN: 1937-3341

Publisher: Mary Ann Liebert

Indexing

ISI Web of Knowledge - 17 Citations

Scopus - 21 Citations

Other information

Authenticus ID: P-003-9MN

DOI: 10.1089/ten.tea.2009.0223

Abstract (EN): In our previous work, biomimetic calcium phosphate-coated poly(caprolactone) nanofibre meshes (BCP-NMs) were demonstrated to be more effective for supporting cell attachment and proliferation under static conditions, when compared with poly(caprolactone) nanofibre meshes (PCL-NMs). In many applications, in vitro cultivation of constructs using bioreactors that support efficient nutrition of cells has appeared as an important step toward the development of functional grafts. This work aimed at studying the effects of dynamic culture conditions and biomimetic coating on bone cells grown on the nanofibre meshes. BCP-NM and PCL-NM were seeded with osteoblast-like cells (MG63-human osteosarcoma-derived cell line). The cell-seeded constructs were cultured within a rotating bioreactor that simulated microgravity, at a fixed rotating speed, for different time periods, and then characterized. Cell morphology, viability, and phenotype were assessed. PCL-NM constructs presented a higher number of dead cells than BCP-NM constructs. Under dynamic conditions, the production of proteins associated with the extracellular matrix of bone was higher on BCP-NM constructs than in the PCL-NM ones, which indicates that coated samples may provide cells with a better environment for tissue growth. It is suggested that improved mass transfer in the bioreactor in combination with the appropriate substrate were decisive factors for this highly positive outcome for generating bone.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7