Cell Adhesion and Proliferation onto Chitosan-based Membranes Treated by Plasma Surface Modification

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Luna, SM (Author) Other; Silva, SS (Author) Other; Gomes, ME (Author) Other; Mano, JF (Author) Other; Rui Reis (Author) Other

Journal

Title: Journal of Biomaterials Applications

Vol. 26

Pages: 101-116

ISSN: 0885-3282

Publisher: SAGE

Indexing

ISI Web of Knowledge - 60 Citations

Scopus - 68 Citations

Other information

Authenticus ID: P-002-Q0A

DOI: 10.1177/0885328210362924

Abstract (EN): Surface properties play a vital role in the functioning of a biomaterial. Cellular adherence and growth onto biomaterials can be enhanced in biomaterial modifications of their surface. In this work, the cell behavior on chitosan membranes modified by argon and nitrogen-plasma treatments was investigated. Characterization of the membranes was performed using atomic force microscopy, contact angle measurements, and X-ray photoelectron spectroscopy. Cytotoxicity assessment and direct contact assay were carried out for untreated and treated chitosan membranes using L929 fibroblast-like cells. Cell morphology and cell viability were assessed to evaluate the cell attachment and proliferation. Changes in terms of roughness, surface chemistry, and hydrophilicity/hydrophobic balance of chitosan-modified membranes were observed. Regarding cell studies, the findings revealed that the extracts of all membranes do not induce cytotoxic effects. Moreover, the in vitro assays evidenced an improvement of the L929 adhesion and attachment when compared to untreated chitosan membranes. Overall, the data obtained clearly demonstrated that plasma treatments constitute an effective way of improving the biocompatibility of chitosan membranes towards to their use in biomedical applications.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 16