Encapsulation of adipose-derived stem cells and transforming growth factor-beta 1 in carrageenan-based hydrogels for cartilage tissue engineering

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Rocha, PM (Author) Other; Santo, VE (Author) Other; Gomes, ME (Author) Other; Rui Reis (Author) Other; Mano, JF (Author) Other

Journal

Title: Journal of Bioactive and Compatible Polymers

Vol. 26

Pages: 493-507

ISSN: 0883-9115

Publisher: SAGE

Indexing

ISI Web of Knowledge - 65 Citations

Scopus - 74 Citations

Other information

Authenticus ID: P-002-N40

DOI: 10.1177/0883911511420700

Abstract (EN): Tissue engineering (TE) is an emerging field for the regeneration of damaged tissues. The combination of hydrogels with stem cells and growth factors (GFs) has become a promising approach to promote cartilage regeneration. In this study, carrageenan-based hydrogels were used to encapsulate both cells and transforming growth factor-beta 1 (TGF-beta 1). The ATDC5 cell line was encapsulated to determine the cytotoxicity and the influence of polymer concentration on cell viability and proliferation. Human adipose-derived stem cells (hASCs) were encapsulated with TGF-beta 1 in the hydrogel networks to enhance the chondrogenic differentiation of hASCs. Specific cartilage extracellular matrix molecules expression by hASCs were observed after 14 days of cultures of the constructs under different conditions. The kappa-carrageenan was found to be a suitable biomaterial for cell and GF encapsulation. The incorporation of TGF-beta 1 within the carrageenan-based hydrogel enhanced the cartilage differentiation of hASCs. These findings indicate that this new system for cartilage TE is very promising for injectable thermoresponsive formulation applications.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 15