Enhancing Dental Pulp Stem Cell Proliferation and Odontogenic Differentiation with Protein Phosphatase 1-Disrupting Peptide: An In Vitro Study

• Type: Article in International Scientific Journal
• Date: 2024

Authors

Kobrock, A (Author) Other; Matos, B (Author) Other; Patricio, D (Author) Other; Liliana Grenho (Author) FMDUP; Howl, J (Author) Other; Fardilha, M (Author) Other; Gomes PS (Author) FMDUP

Journal

Title: Cells

Vol. 13

Final page: 1143

ISSN: 2073-4409

Publisher: MDPI

Indexing

ISI Web of Knowledge - 0 Citations

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Other information

Authenticus ID: P-010-YZJ

DOI: 10.3390/cells13131143

Abstract (EN): The reparative and regenerative capabilities of dental pulp stem cells (DPSCs) are crucial for responding to pulp injuries, with protein phosphatase 1 (PP1) playing a significant role in regulating cellular functions pertinent to tissue healing. Accordingly, this study aimed to explore the effects of a novel cell-penetrating peptide Modified Sperm Stop 1-MSS1, that disrupts PP1, on the proliferation and odontogenic differentiation of DPSCs. Employing MSS1 as a bioportide, DPSCs were cultured and characterized for metabolic activity, cell proliferation, and cell morphology alongside the odontogenic differentiation through gene expression and alkaline phosphatase (ALP) activity analysis. MSS1 exposure induced early DPSC proliferation, upregulated genes related to odontogenic differentiation, and increased ALP activity. Markers associated with early differentiation events were induced at early culture time points and those associated with matrix mineralization were upregulated at mid-culture stages. This investigation is the first to document the potential of a PP1-disrupting bioportide in modulating DPSC functionality, suggesting a promising avenue for enhancing dental tissue regeneration and repair.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9