Optimized reconstruction of the absorption spectra of kidney tissues from the spectra of tissue components using the least squares method

• Type: Article in International Scientific Journal
• Date: 2024

Authors

Pinheiro, MR (Author) Other; Fernandes, LE (Author) Other; Carneiro, IC (Author) Other; Carvalho, SD (Author) Other; Henrique, RM (Author) Other; Tuchin, VV (Author) Other; Oliveira, HP (Author) FCUP; Oliveira, LM (Author) Other

Journal

Title: Journal of Biophotonics

ISSN: 1864-063X

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 3 Citations

Other information

Authenticus ID: P-00Z-ZEP

DOI: 10.1002/jbio.202300466

Abstract (EN): With the objective of developing new methods to acquire diagnostic information, the reconstruction of the broadband absorption coefficient spectra (mu a[lambda]) of healthy and chromophobe renal cell carcinoma kidney tissues was performed. By performing a weighted sum of the absorption spectra of proteins, DNA, oxygenated, and deoxygenated hemoglobin, lipids, water, melanin, and lipofuscin, it was possible to obtain a good match of the experimental mu a(lambda) of both kidney conditions. The weights used in those reconstructions were estimated using the least squares method, and assuming a total water content of 77% in both kidney tissues, it was possible to calculate the concentrations of the other tissue components. It has been shown that with the development of cancer, the concentrations of proteins, DNA, oxygenated hemoglobin, lipids, and lipofuscin increase, and the concentration of melanin decreases. Future studies based on minimally invasive spectral measurements will allow cancer diagnosis using the proposed approach.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10