Microbacterium memoriense sp. nov., a member of the Actinomycetota from marine beach sediment of the north coast of Portugal

• Type: Article in International Scientific Journal
• Date: 2024

Authors

Santos, JDND (Author) Other; Klimek, D (Author) Other; Calusinska, M (Author) Other; Lobo-da-Cunha, A (Author) Other; Catita, J (Author) Other; Gonçalves, H (Author) Other; González, I (Author) Other; lage, om (Author) FCUP

Journal

Title: International Journal of Systematic and Evolutionary Microbiology

Vol. 74

ISSN: 1466-5026

Publisher: Microbiology Society

Indexing

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Other information

Authenticus ID: P-00Z-RNB

DOI: 10.1099/ijsem.0.006230

Abstract (EN): The oceans harbour a myriad of unknown micro- organisms that remain unstudied because of a failure to establish the right growth conditions under laboratory conditions. To overcome this limitation, an isolation effort inspired by the iChip was performed using marine sediments from Memoria beach, Portugal. A novel strain, PMIC_1C1BT, was obtained and subjected to a polyphasic study. Cells of strain PMIC_1C1BT were Gram- positive, rod- shaped, divided by binary fission and formed colonies that were shiny light- yellow. Based on its full 16S rRNA gene sequence, strain PMIC_1C1BT was phylogenetically associated to the genus Microbacterium and its closest relatives were Microbacterium aurum KACC 15219T (98.55 %), Microbacterium diaminobutyricum RZ63T (98.48 %) and Microbacterium hatanonis JCM 14558T (98.13 %). Strain PMIC_1C1BT had a genome size of 2 761 607 bp with 67.71 mol% of G+C content and 2582 coding sequences, which is lower than the genus average. Strain PMIC_1C1BT grew from 15 to 30 degrees C, optimally at 25 degrees C, at pH 6.0 to 11.0, optimally between pH 6.0 and 8.0, and from 0 to 5 % (w/v) NaCl, optimally between 2.0 and 3.0 %. It grew with casamino acids, glutamine, methionine, N-acetylglucosamine, sodium nitrate, tryptophan, urea and valine as sole nitrogen sources, and arabinose and cellobiose as sole carbon sources. The major cellular fatty acids were anteiso- C15:0, iso-C16: 0 and iso- C17:0. Genome mining revealed the presence of four biosynthetic gene clusters (BGCs) with low similarities to other known BCGs. Based on the polyphasic data, strain PMIC_1C1BT is proposed to represent a novel species, for which the name Microbacterium memoriense sp. nov. (=CECT 30366T=LMG 32350T) is proposed.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12