Antimicrobial Resistance Profile of Planctomycetota Isolated from Oyster Shell Biofilm: Ecological Relevance within the One Health Concept

• Type: Article in International Scientific Journal
• Date: 2024

Authors

Guedes, B (Author) Other; Godinho, O (Author) Other; Quinteira, S (Author) FCUP; lage, om (Author) FCUP

Journal

The Journal is awaiting validation by the Administrative Services.

Title: Applied Microbiology

Vol. 4

Pages: 16-26

ISSN: 2673-8007

Indexing

Scopus - 0 Citations

Other information

Authenticus ID: P-010-H76

DOI: 10.3390/applmicrobiol4010002

Abstract (EN): Background: Planctomycetota isolation in pure culture is still challenging with most of the reported data coming from molecular-based methods. Here, we intended to isolate Planctomycetota from the filter-feeder Pacific oyster Magallana gigas, extending the search to a not yet explored natural reservoir and to characterize their antimicrobial resistance phenotype. Methods: Oyster samples from different supermarkets and from a farm producer were subject to isolation in selective medium. Inoculation was performed from the shell biofilm and after an enrichment of the edible content. Results: Planctomycetota isolates (n = 65) were only obtained from the shell biofilm with four different species identified: Rhodopirellula baltica (n = 62), Rhodopirellula rubra (n = 1), Rhodopirellula heiligendammensis (n = 1) and Gimesia chilikensis (n = 1). This study reports the first association of Planctomycetota members with oysters and the first description of R. heiligendammensis in Portugal. Moreover, R. rubra, originally identified in Portugal, was isolated from oysters of French origin. Antibiotic susceptibility testing, conducted in strains belonging to two species never assayed before revealed multidrug resistance phenotypes with bacteria showing resistance to several classes of clinically relevant antibiotics (e.g., ß-lactams and aminoglycosides). Conclusion: The ecological role and impact of Planctomycetota on oyster holobiont and, ultimately, in public health, under the One Health concept, is discussed. © 2023 by the authors.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10