Immunostimulant properties of full-length and truncated Marinobacter algicola flagellins, and their effects against viral hemorrhagic septicemia virus (VHSV) in trout

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Nunez Ortiz, N (Author) Other; Diaz Rosales, P (Author) Other; Garcia, JA (Author) Other; Serra, CR (Author) Other; Enes, P (Author) FCUP; Tafalla, C (Author) Other; Gomez Casado, E (Author) Other

Journal

Title: Fish and Shellfish Immunology

Vol. 128

Pages: 695-702

ISSN: 1050-4648

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 1 Citation

Scopus - 2 Citations

Other information

Authenticus ID: P-00X-91M

DOI: 10.1016/j.fsi.2022.08.018

Abstract (EN): Adjuvants that would help optimize fish vaccines against bacterial and viral pathogens are highly demanded by the aquaculture sector. Flagellin has been proposed as an immunostimulant and an adjuvant for more than a decade. However, the adjuvant ability of flagellins with hypervariable region deleted is still unclear in fish. In this study, we evaluated the immune-stimulating capacity of two recombinant flagellins, the wild-type flagellin F from Marinobacter algicola and a version with the hypervariable region deleted (FredV2), to induce the transcription of a wide range of immune genes using two rainbow trout cell lines: a monocyte/macrophage-cell line (RTS-11) and an epithelial cell line from intestine (RTgutGC). Additionally, we studied the capacity of both flagellins to limit the replication of viral hemorrhagic septicemia virus (VHSV) on the RTgutGC cell line. Our results demonstrated that both recombinant flagellins can significantly increase the transcription of IL-1 beta 1, IL-6, and IL-8 in both cell lines. However, other cytokines such as IFN gamma 1, and TNF alpha or antimicrobial peptides such as hepcidin were induced by both flagellins in RTgutGC but not in RTS-11 cells. Furthermore, both flagellins were capable of reducing the replication of VHSV in RTgutGC cells. Although the immunostimulatory and the antiviral capacities exerted by F were slightly more potent than those obtained with FredV2, the effects were retained after losing the hypervariable region. Our results provide new information on the immunostimulating and antiviral capacities of flagellins that point to their potential as suitable adjuvants for the future optimization of vaccines in aquaculture.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8