SYNTHETIC BIOLOGY IN CYANOBACTERIA: ENGINEERING AND ANALYZING NOVEL FUNCTIONS

• Type: Another Publication in an International Scientific Journal
• Date: 2011

Authors

Heidorn, T (Author) Other; Camsund, D (Author) Other; Huang, HH (Author) Other; Lindberg, P (Author) Other; Oliveira, P (Author) Other; Stensjo, K (Author) Other; Lindblad, P (Author) Other

Journal

The Journal is awaiting validation by the Administrative Services.

Title: METHODS IN ENZYMOLOGY, VOL 497: SYNTHETIC BIOLOGY, METHODS FOR PART/DEVICE CHARACTERIZATION AND CHASSIS ENGINEERING, PT A

Vol. 16

Pages: 539-579

ISSN: 0076-6879

Publisher: ELSEVIER ACADEMIC PRESS INC

Indexing

ISI Web of Knowledge - 34 Citations

Scopus - 199 Citations

Other information

Authenticus ID: P-009-FHP

DOI: 10.1016/b978-0-12-385075-1.00024-x

Abstract (EN): Cyanobacteria are the only prokaryotes capable of using sunlight as their energy, water as an electron donor, and air as a source of carbon and, for some nitrogen-fixing strains, nitrogen. Compared to algae and plants, cyanobacteria are much easier to genetically engineer, and many of the standard biological parts available for Synthetic Biology applications in Escherichia coli can also be used in cyanobacteria. However, characterization of such parts in cyanobacteria reveals differences in performance when compared to E. coli, emphasizing the importance of detailed characterization in the cellular context of a biological chassis. Furthermore, cyanobacteria possess special characteristics (e.g., multiple copies of their chromosomes, high content of photosynthetically active proteins in the thylakoids, the presence of exopolysaccharides and extracellular glycolipids, and the existence of a circadian rhythm) that have to be taken into account when genetically engineering them. With this chapter, the synthetic biologist is given an overview of existing biological parts, tools and protocols for the genetic engineering, and molecular analysis of cyanobacteria for Synthetic Biology applications.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 41