Characterization of ten H-2 producing cyanobacteria isolated from the Baltic Sea and Finnish lakes

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Leino, H (Author) Other; Shunmugam, S (Author) Other; Isojarvi, J (Author) Other; Oliveira, P (Author) Other; Mulo, P (Author) Other; Saari, L (Author) Other; Battchikova, N (Author) Other; Sivonen, K (Author) Other; Lindblad, P (Author) Other; Aro, EM (Author) Other; Allahverdiyeva, Y (Author) Other

Journal

Title: International Journal of Hydrogen Energy

Vol. 39 No. 25

Pages: 8983-8991

ISSN: 0360-3199

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 21 Citations

Other information

Authenticus ID: P-009-DQY

DOI: 10.1016/j.ijhydene.2014.03.171

Abstract (EN): The genetic background and activities of the enzymes involved in H-2 production were investigated from ten distinct H-2 producing cyanobacteria, revealed by a recent screening. All strains are N-2-fixing, filamentous and heterocystous. Southern hybridization revealed that the tested strains possess the genes encoding the conventional nitrogenase (nifHDK1), and lack the alternative nitrogenases. The high H-2 production rate of these strains was shown not to be dependent on the presence of highly active nitrogenase or bidirectional hydrogenase enzymes. Moreover, most of the strains possessed a highly active uptake hydrogenase enzyme. We also examined the structure of the nif and hup operons encoding nitrogenase and uptake hydrogenase enzymes in the Calothrix 336/3 strain, the best H-2 producer in the screening. We concluded that the ability of the cyanobacteria to produce high levels of H-2 is not directly linked to the maximum capacities of the enzymes involved in H-2 production. Copyright

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9