LexA, a transcription regulator binding in the promoter region of the bidirectional hydrogenase in the cyanobacterium Synechocystis sp PCC 6803

• Type: Article in International Scientific Journal
• Date: 2005

Authors

Oliveira, P (Author) Other; Lindblad, P (Author) Other

Journal

Title: FEMS Microbiology Letters

Vol. 251

Pages: 59-66

ISSN: 0378-1097

Publisher: Oxford University Press

Indexing

ISI Web of Knowledge - 40 Citations

Scopus - 60 Citations

Other information

Authenticus ID: P-00F-RG8

DOI: 10.1016/j.femsle.2005.07.024

Abstract (EN): The unicellular cyanobacterium Synechocystis PCC 6803 contains a single pentameric bidirectional hydrogenase encoded by hox-EFUYH. Transcriptional experiments demonstrated that the five hox genes are part of a single transcript together with three ORFs with unknown functions. The transcription start point was localized by 5' RACE to 168bp upstream the hoxE ATG start codon. DNA affinity assays demonstrated a specific interaction between the hox regulatory promoter region and a protein which, using mass spectrometry, was identified to be LexA. Overexpressed His-tagged Synechocystis LexA and EMSA showed a specific binding to the promoter region of the hox operon. Increasing concentrations of the purified LexA resulted in two retarded LexA-DNA complexes, in agreement with the presence of two putative LexA binding sites upstream the determined TSP.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8