Desorption of cellulases from cotton powder

• Type: Article in International Scientific Journal
• Date: 2001

Authors

Azevedo, H (Author) Other; Ramos, LP (Author) Other; Cavaco Paulo, A (Author) Other

Journal

Title: Biotechnology Letters

Vol. 23

Pages: 1445-1448

ISSN: 0141-5492

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 10 Citations

Scopus - 12 Citations

Other information

Authenticus ID: P-000-TBF

DOI: 10.1023/a:1011619425613

Abstract (EN): Cotton fabrics were treated with three different Trichoderma reesei cellulase preparations (total crude - TC, endoglucanase enriched - EG-rich, cellobiohydrolase enriched - CBH-rich) using mechanical agitation to produce cotton powder. Desorption of cellulase enzymes from the cotton powder was then performed by washing with buffer. After 3 washings most of the protein was desorbed from the cotton powder and the amount of sugars released in the latter washings was negligible. TC and CBH-rich preparations produced a finer cellulose powder than EGs. The desorption process caused a decrease in degree of polymerisation (DP) specially for the cotton treated with EGs and a marked increase in polydispersity (P-d) for all preparations.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4