Analysis on the Role of Phenylpropanoid Metabolism in the Pinus pinaster-Botrytis cinerea Interaction

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Azevedo, H (Author) Other; Dias, A (Author) Other; Tavares, RM (Author) Other

Journal

The Journal is awaiting validation by the Administrative Services.

Title: JOURNAL OF PHYTOPATHOLOGY

Vol. 158

Pages: 641-646

ISSN: 0931-1785

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 6 Citations

Other information

Authenticus ID: P-003-3N6

DOI: 10.1111/j.1439-0434.2010.01673.x

Abstract (EN): The interaction between maritime pine (Pinus pinaster) and the necrotrophic pathogen Botrytis cinerea was addressed at the level of phenylpropanoid metabolism using a suspension cell model system. HPLC-DAD analysis revealed the presence of several phenolic compounds, including derivatives of epicatechin, caffeoylquinic acid and glycosylated quercetin. However, challenged cells evidenced a reduction in total soluble phenolics and a decrease in the lignin content of cells. Key phenylpropanoid metabolism genes Pal and Chs were isolated after screening a P. pinaster cDNA library. Expression analysis evidenced a downregulation of Pal transcripts. Chs transcripts were observed in P. pinaster needles but not in suspension cells. With regard to the P. pinaster-B. cinerea interaction, results indicate that elicited cells downregulate phenylpropanoid metabolism, which suggests that systemic acquired resistance is not induced after challenging with this necrotrophic pathogen.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6