Establishment and characterization of Pinus pinaster suspension cell cultures

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Azevedo, H (Author) Other; Dias, A (Author) Other; Tavares, RM (Author) Other

Journal

Title: Plant Cell, Tissue and Organ Culture

Vol. 93

Pages: 115-121

ISSN: 0167-6857

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 13 Citations

Other information

Authenticus ID: P-004-0Q2

DOI: 10.1007/s11240-008-9349-1

Abstract (EN): We report the establishment of a Pinus pinaster (Ait.) cell suspension culture in a modified MS medium supplemented with 2 mg ml(-1) 2,4-D and 1 mg ml(-1) BA. Calli were obtained from seedling root segments and established a friable isodiametric cell suspension, suitable for in vitro studies of maritime pine at the cellular level. Growth (dry weight), cell viability, pH, and nutrient consumption: carbon source (sucrose, fructose and glucose), nitrogen source (ammonia and nitrate) and phosphate were monitored over 24 h. Suspension cells exhibited a 15-day exponential growth stage, during which a biphasic consumption profile was observed for all nutrients. Phosphate was the first limiting nutrient and preferable consumption was observed for glucose over fructose and nitrate over ammonium.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7