Differences Amid Bone Marrow and Cord Blood Hematopoietic Stem/Progenitor Cell Division Kinetics

• Type: Article in International Scientific Journal
• Date: 2009

Authors

Da Silva, CL (Author) Other; Goncalves, R (Author) Other; Porada, CD (Author) Other; Ascensao, JL (Author) Other; Zanjani, ED (Author) Other; Cabral, JMS (Author) Other; Almeida Porada, G (Author) Other

Journal

Title: Journal of Cellular Physiology

Vol. 220

Pages: 102-111

ISSN: 0021-9541

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 36 Citations

Scopus - 36 Citations

Other information

Authenticus ID: P-007-Q4K

DOI: 10.1002/jcp.21736

Abstract (EN): Human hematopoietic stem/progenitor cells (HSC) isolated based upon specific patterns of CD34 and CD38 expression, despite phenotypically identical, were found to be functionally heterogeneous, raising the possibility that reversible expression of these antigens may occur during cellular activation and/or proliferation. In these studies, we combined PKH67 tracking with CD34/CD38 immunostaining to compare cell division kinetics between human bone marrow (BM) and cord blood (CB)-derived HSC expanded in a serum-free/stromal-based system for 14 days (d), and correlated CD34 and CD38 expression with the cell divisional history. CB cells began dividing 24 h earlier than BM cells, and significantly higher numbers underwent mitosis during the time in culture. By d10, over 55% of the CB-cells reached the ninth generation, whereas BM-cells were mostly distributed between the fifth and seventh generation. By d14, all CB cells had undergone multiple cell divisions, while 0.7-3.8% of BM CD34(+) cells remained quiescent. Furthermore, the percentage of BM cells expressing CD34 decreased from 60.8 +/- 6.3% to 30.6 +/- 6.7% prior to initiating division, suggesting that downmodulation of this antigen occurred before commencement of proliferation. Moreover, with BM, all primitive CD34(+) CD38(-) cells present at the end of culture arose from proliferating CD34(+) CD38(+); cells that downregulated CD38 expression, while in CB, a CD34(+) CD38(-) population was maintained throughout culture. These studies show that BM and CB cells differ significantly in cell division kinetics and expression of CD34 and CD38, and that the inherent modulation of these antigens during ex vivo expansion may lead to erroneous quantification of the stem cell content of the expanded graft. J. Cell. Physiol. 220: 102-111, 2009. (C) 2009 Wiley-Liss, Inc.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10