Kinetic analysis of the ex vivo expansion of human hematopoietic stem/progenitor cells

• Type: Article in International Scientific Journal
• Date: 2006

Authors

Goncalves, R (Author) Other; da Silva, CL (Author) Other; Ferreira, BS (Author) Other; Lemos, MANDA (Author) Other; Lemos, F (Author) Other; Almeida Porada, G (Author) Other; Cabral, JMS (Author) Other

Journal

Title: Biotechnology Letters

Vol. 28

Pages: 335-340

ISSN: 0141-5492

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 7 Citations

Scopus - 8 Citations

Other information

Authenticus ID: P-004-MVZ

DOI: 10.1007/s10529-005-5932-2

Abstract (EN): The total cell expansion of human umbilical cord blood (CB) and adult bone marrow (BM) CD34(+)-enriched cells cultured in supplemented serum-free media, either over irradiated human feeder layers or in stroma-free systems, were characterized by a simple kinetic model using only two parameters: the specific cell expansion rate, mu, and the death rate constant, k(k). Both CB and BM cells can expand at approximately the same rate (0.21 day(-1)) in this culture system however, cell death depends on the presence of stroma and the environment in which the cells are cultured.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6