A Stro-1(+) human universal stromal feeder layer to expand/maintain human bone marrow hematopoietic stem/progenitor cells in a serum-free culture system

• Type: Article in International Scientific Journal
• Date: 2006

Authors

Goncalves, R (Author) Other; da Silva, CL (Author) Other; Cabral, JMS (Author) Other; Zanjani, ED (Author) Other; Almeida Porada, G (Author) Other

Journal

Title: Experimental Hematology

Vol. 34

Pages: 1353-1359

ISSN: 0301-472X

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 53 Citations

Scopus - 60 Citations

Other information

Authenticus ID: P-004-GT1

DOI: 10.1016/j.exphem.2006.05.024

Abstract (EN): Objective. To compare the ability of allogeneic versus autologous purified human Stro-1(+) mesenchymal stem cell (MSC) populations from different human donors to support the ex vivo expansion and maintenance of human hematopoietic stem/progenitor cells (HSCs). Furthermore, we compared the results obtained with MSC as a feeder layer to traditional allogeneic stromal layers grown in long-term bone marrow culture media (LT-ST). Methods. Adult human bone marrow CD34(+)-enriched cells were cultured in serum-free medium for 2 to 3 weeks over the respective MSC-irradiated feeder layers or over traditional allogeneic LT-ST stromal layers in the presence of stem cell factor, basic fibroblast growth factor, leukemia inhibitory factor, and Flt-3 and analyzed every 2 to 4 days for expansion, phenotype, and clonogenic ability. Results. There was a progressive expansion of total numbers of cells in all the experimental groups; however, allogeneic MSCs were more efficient at expanding CD34(+)CD38(-) cells and showed a higher clonogenic potential than both allogeneic LT-ST and autologous MSCs. The differentiative potential of cells cultured on both MSC and LT-ST was primarily shifted toward myeloid lineage; however, only MSCs were able to maintain/expand a CD7(+) population with lymphocytic potential. Importantly, transplantation into preimmune fetal sheep demonstrated that the HSCs cultured over MSCs retained their engraftment capability. Conclusion. These results indicate that purified Stro-1(+) MSCs may be used as a universal and reproducible stromal feeder layer to efficiently expand and maintain human bone marrow HSCs ex vivo. (c) 2006 International Society for Experimental Hematology. Published by Elsevier Inc.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7