Hematopoietic stem cells: from the bone to the bioreactor

• Type: Another Publication in an International Scientific Journal
• Date: 2003

Authors

Cabrita, GJM (Author) Other; Ferreira, BS (Author) Other; da Silva, CL (Author) Other; Goncalves, R (Author) Other; Almeida Porada, G (Author) Other; Cabral, JMS (Author) Other

Journal

Title: Trends in Biotechnology

Vol. 21

Pages: 233-240

ISSN: 0167-7799

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 95 Citations

Scopus - 110 Citations

Other information

Authenticus ID: P-000-H0G

DOI: 10.1016/s0167-7799(03)00076-3

Abstract (EN): The ex vivo expansion of human hematopoietic stem cells is a rapidly developing area with a broad range of biomedical applications. The mechanisms of renewal, differentiation and plasticity of stem cells are currently under intense investigation. However, the complexity of hematopoiesis, the heterogeneity of the culture population and the complex interplay between the culture parameters that significantly influence the proliferation and differentiation of hematopoietic cells have impaired the translation of small scale results to the highly demanded large-scale applications. The better understanding of these mechanisms is providing the basis for more rational approaches to the ex vivo expansion of hematopoietic stem cells. Efforts are now being made to establish a rational design of bioreactor systems, allowing the modeling and control of large-scale production of stem cells and the study of their proliferation and differentiation, under conditions as similar as possible to those in vivo.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8