A human stromal-based serum-free culture system supports the ex vivo expansion/maintenance of bone marrow and cord blood hematopoietic stem/progenitor cells

• Type: Article in International Scientific Journal
• Date: 2005

Authors

da Silva, CL (Author) Other; Goncalves, R (Author) Other; Crapnell, KB (Author) Other; Cabral, JMS (Author) Other; Zanjani, ED (Author) Other; Almeida Porada, G (Author) Other

Journal

Title: Experimental Hematology

Vol. 33

Pages: 828-835

ISSN: 0301-472X

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 88 Citations

Scopus - 101 Citations

Other information

Authenticus ID: P-000-2HB

DOI: 10.1016/j.exphem.2005.03.017

Abstract (EN): Objective. We investigated the role of human stromal layers (hu-ST) on the ex vivo expansion/maintenance of human hematopoietic stem/progenitor cells (HSC) from adult bone marrow (BM) and umbilical cord blood (CB). Materials and Methods. BM and CB CD34(+)-enriched cells were cultured in serum-free medium supplemented with SCF, bFGF, LIF, and Flt-3, in the presence or absence of stroma, and analyzed for proliferation, phenotype, and clonogenic potential. Results. Significant expansion of BM and CB CD34(+) and CD34(+)CD38(-) cells were achieved in the presence of hu-ST. The differentiative potential of both BM and CB CD34+-enriched cells cocultured with hu-ST was primarily shifted toward the myeloid lineage, while maintaining/ expanding a CD7(+) population. Clonogenic analysis of the expanded cells showed increases in progenitors of the myeloid lineage, including colony-forming unit-granulocyte, macrophage (CFU-GM) and colony-forming unit-granulocyte, erythroid, macrophage, megakaryocyte (CFU-Mix) for both BM (stroma and stroma-free conditions) and CB cells in the presence of stroma. Conclusions. These results indicate that adult hu-ST in the presence of appropriate cytokines can be used to efficiently expand/maintain myeloid and lymphoid cell populations from human BM and CB HSC. (c) 2005 International Society for Experimental Hematology. Published by Elsevier Inc.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8