Characterization of an antioxidant surfactant-free topical formulation containing Castanea sativa leaf extract

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Isabel F Almeida (Author) FFUP; Jitka Maleckova (Author) Other; Raquel Saffi (Author) Other; Helena Monteiro (Author) Other; Filipa Goios (Author) Other; Maria Helena Amaral (Author) FFUP; Paulo Costa (Author) FFUP; Jorge Garrido (Author) Other; Paulo Silva (Author) FFUP; Nazare Pestana (Author) Other; M.F. Bahia (Author) FFUP

Journal

Title: Drug Development and Industrial Pharmacy

Vol. 41

Pages: 148-155

ISSN: 0363-9045

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 17 Citations

Scopus - 21 Citations

Pubmed / Medline

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-00A-19F

DOI: 10.3109/03639045.2013.850712

Abstract (EN): Context: Inclusion of antioxidants in topical formulations can contribute to minimize oxidative stress in the skin, which has been associated with photoaging, several dermatosis and cancer. Objective: A Castanea sativa leaf extract with established antioxidant activity was incorporated into a semisolid surfactant-free formulation. The objective of this study was to perform a comprehensive characterization of this formulation. Materials and methods: Physical, microbiological and functional stability were evaluated during 6 months storage at 20 degrees C and 40 degrees C. Microstructure elucidation (cryo-SEM), in vitro release and in vivo moisturizing effect (Corneometer (R) CM 825) were also assessed. Results and discussion: Minor changes were observed in the textural and rheological properties of the formulation when stored at 20 degrees C for 6 months and the antioxidant activity of the plant extract remained constant throughout the storage period. Microbiological quality was confirmed at the end of the study. Under accelerated conditions, higher modifications of the evaluated parameters were observed. Cryo-SEM analysis revealed the presence of oil droplets dispersed into a gelified external phase. The release rate of the antioxidant compounds (610 +/- 70 mu gh(-0.5)) followed Higuchi model. A significant in vivo moisturizing effect was demonstrated, that lasted at least 4 h after product's application. Conclusion: The physical, functional and microbiological stability of the antioxidant formulation was established. Specific storage conditions should be recommended considering the influence of temperature on the stability. A skin hydration effect and good skin tolerance were also found which suggests that this preparation can be useful in the prevention or treatment of oxidative stress-mediated dysfunctions.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8