OXA-23-producing Acinetobacter baumannii: a new hotspot of diversity in Rio de Janeiro?

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Filipa Grosso (Author) Other; Karyne Rangel Carvalho (Author) Other; Sandra Quinteira (Author) Other; Ana Ramos (Author) Other; Ana Paula D D'Alincourt Carvalho Assef (Author) Other; Marise Dutra Asensi (Author) Other; Luisa Peixe (Author) FFUP

Journal

Title: Journal of Antimicrobial Chemotherapy

Vol. 66 No. 9

Pages: 62-65

ISSN: 0305-7453

Publisher: Oxford University Press

Indexing

ISI Web of Knowledge - 51 Citations

ISI Web of Science

Scopus - 55 Citations

Pubmed / Medline

Scientific classification

FOS: Medical and Health sciences > Health sciences

Other information

Authenticus ID: P-002-YWV

DOI: 10.1093/jac/dkq406

Resumo (PT): Objectives This study focused on the population structure of OXA-23-producing Acinetobacter baumannii clinical isolates from Rio de Janeiro, Brazil. Methods The analysis included several genomic typing methods, including PFGE, two multilocus sequence typing (MLST) schemes, sequence group (SG) determination and blaOXA-51-like sequencing. The genomic context of the blaOXA-23 gene was also evaluated using I-CeuI hybridizations and PCR assays. Results Congruent clustering was obtained revealing four lineages. In accordance, four new sequence types (STs) (ST131, ST132, ST133 and ST134) were obtained with the MLST-OD scheme (associated with the Oxford Database) and four (ST79, ST15 and two new allelic profiles) with the MLST-IP scheme (developed by the Institute Pasteur). Four SGs (SG1, SG4 and two new profiles) were identified, allowing the association of 70% of the isolates with European clone II. blaOXA-51-like sequencing revealed the presence of blaOXA-66, blaOXA-69, blaOXA-95 and blaOXA-132. Conclusions Identification of new STs together with new SG profiles are findings suggestive of a local diversity hotspot that is worth exploring <br> <br> Key words blaOXA-23PFGEMLSTsequence groupsblaOXA-51-like <br> <a target="_blank" href="http://jac.oxfordjournals.org/content/66/1/62.full"> texto integral </a> <br> <br>

Abstract (EN): This study focused on the population structure of OXA-23-producing Acinetobacter baumannii clinical isolates from Rio de Janeiro, Brazil. The analysis included several genomic typing methods, including PFGE, two multilocus sequence typing (MLST) schemes, sequence group (SG) determination and bla(OXA-51-like) sequencing. The genomic context of the bla(OXA-23) gene was also evaluated using I-CeuI hybridizations and PCR assays. Congruent clustering was obtained revealing four lineages. In accordance, four new sequence types (STs) (ST131, ST132, ST133 and ST134) were obtained with the MLST-OD scheme (associated with the Oxford Database) and four (ST79, ST15 and two new allelic profiles) with the MLST-IP scheme (developed by the Institute Pasteur). Four SGs (SG1, SG4 and two new profiles) were identified, allowing the association of 70% of the isolates with European clone II. bla(OXA-51-like) sequencing revealed the presence of bla(OXA-66), bla(OXA-69), bla(OXA-95) and bla(OXA-132). Identification of new STs together with new SG profiles are findings suggestive of a local diversity hotspot that is worth exploring.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4