Development of a Multicommutated Flow System with Chemiluminometric Detection for Quantification of Gentamicin in Pharmaceuticals

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Lucia H M L M Santos (Author) Other; Araujo, AN (Author) FFUP; Boaventura Reis (Author) Other; Montenegro, MCBSM (Author) FFUP

Journal

Title: Journal of Automated Methods and Management in Chemistry

Vol. 2010

Pages: 1-7

ISSN: 1463-9246

Publisher: HINDAWI PUBLISHING CORPORATION

Indexing

ISI Web of Knowledge - 2 Citations

ISI Web of Science

Scopus - 3 Citations

Pubmed / Medline

Scientific classification

CORDIS: Health sciences > Pharmacological sciences > Pharmacy

FOS: Engineering and technology > Other engineering and technologies

Other information

Authenticus ID: P-003-BJ6

DOI: 10.1155/2010/913207

Resumo (PT): A new flow procedure based on multicommutation with chemiluminometric detection was developed to quantify gentamicin sulphate in pharmaceutical formulations. This approach is based on gentamicin's ability to inhibit the chemiluminometric reaction between luminol and hypochlorite in alkaline medium, causing a decrease in the analytical signal. The inhibition of the analytical signal is proportional to the concentration of gentamicin sulphate, within a linear range of 1 to 4 μg mL-1 with a coefficient variation <3%. A sample throughput of 55 samples h-1 was obtained. The developed method is sensitive, simple, with low reagent consumption, reproducible, and inexpensive, and when applied to the analysis of pharmaceutical formulations (eye drops and injections) it gave results with RSD between 1.10 and 4.40%. <br> <br> <a target="_blank" href="http://www.hindawi.com/journals/jammc/2010/913207/ "> Texto integral </a> <br> <br>

Abstract (EN): A new flow procedure based on multicommutation with chemiluminometric detection was developed to quantify gentamicin sulphate in pharmaceutical formulations. This approach is based on gentamicin's ability to inhibit the chemiluminometric reaction between luminol and hypochlorite in alkaline medium, causing a decrease in the analytical signal. The inhibition of the analytical signal is proportional to the concentration of gentamicin sulphate, within a linear range of 1 to 4 mu g mL(-1) with a coefficient variation <3%. A sample throughput of 55 samples h(-1) was obtained. The developed method is sensitive, simple, with low reagent consumption, reproducible, and inexpensive, and when applied to the analysis of pharmaceutical formulations (eye drops and injections) it gave results with RSD between 1.10 and 4.40%.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7