Effects induced by the nodulation with Bradyrhizobium japonicum on Glycine max (soybean) metabolism and antioxidant potential

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Carla Couto (Author) Other; Luis R Silva (Author) Other; Patricia Valentao (Author) FFUP; Encarna Velazquez (Author) Other; Alvaro Peix (Author) Other; Paula B Andrade (Author) FFUP

Journal

Title: Food Chemistry

Vol. 127 No. 4

Pages: 1487-1495

ISSN: 0308-8146

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 18 Citations

Scopus - 25 Citations

Scientific classification

FOS: Agrarian Sciences > Other Agrarian Sciences

CORDIS: Health sciences > Pharmacological sciences > Pharmacognosy

Other information

Authenticus ID: P-002-NR3

DOI: 10.1016/j.foodchem.2011.01.135

Resumo (PT): This study was conducted in order to assess the effect of the inoculation of Glycine max (soybean) with Bradyrhizobium japonicum on the metabolite profile and antioxidant potential of its aerial parts. Extracts containing phenolic compounds and organic acids were analysed by HPLC–DAD and HPLC–UV, respectively. Volatiles profile was determined by HS-SPME/GC–IT-MS. The antioxidant activity of acidic and methanolic extracts was assessed against DPPH. Nodulation caused by B. japonicum led to an increase in the contents of phenolic compounds and organic acids. The same was observed for volatile compounds, with compounds like linalyl acetate, menthyl acetate and α-farnesene being detected only in inoculated plants. The phenolic extracts showed stronger antioxidant capacity than the organic acid extracts. In addition, extracts from plants nodulated with B. japonicum exhibited significantly higher antioxidant activity than control samples. These findings suggest that the inoculation with nodulating B. japonicum strains may be employed to manipulate the content of interesting metabolites in G. max aerial parts. <br> <br> Keywords: Glycine max; Bradyrhizobium japonicum; Volatile compounds; Organic acids; Phenolic compounds; Antioxidant capacity <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T6R-523V3K6-3&_user=2460038&_coverDate=08%2F15%2F2011&_rdoc=11&_fmt=high&_orig=browse&_origin=browse&_zone=rslt_list_item&_srch=doc-info(%23toc%235037%232011%23998729995%233044776%23FLA%23display%23Volume)&_cdi=5037&_sort=d&_docanchor=&_ct=75&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=e0f92208bccef1d459af8d165974f547&searchtype=a "> Texto integral </a> <br> <br>

Abstract (EN): This study was conducted in order to assess the effect of the inoculation of Glycine max (soybean) with Bradyrhizobium japonicum on the metabolite profile and antioxidant potential of its aerial parts. Extracts containing phenolic compounds and organic acids were analysed by HPLC-DAD and HPLC-UV, respectively. Volatiles profile was determined by HS-SPME/GC-IT-MS. The antioxidant activity of acidic and methanolic extracts was assessed against DPPH. Nodulation caused by B. japonicum led to an increase in the contents of phenolic compounds and organic acids. The same was observed for volatile compounds, with compounds like linalyl acetate, menthyl acetate and alpha-farnesene being detected only in inoculated plants. The phenolic extracts showed stronger antioxidant capacity than the organic acid extracts. In addition, extracts from plants nodulated with B. japonicum exhibited significantly higher antioxidant activity than control samples. These findings suggest that the inoculation with nodulating B. japonicum strains may be employed to manipulate the content of interesting metabolites in G. max aerial parts.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9