Enzymatic Determination of Glucose in Milk Samples by Sequential Injection Analysis

• Type: Article in International Scientific Journal
• Date: 2009

Authors

Alberto N Araujo (Author) FFUP; Jose L F C Lima (Author) FFUP; Paula C A G Pinto (Author) FFUP; Lucia L M F S Saraiva (Author) FFUP

Journal

Title: Analytical Sciences

Vol. 25 No. 5

Pages: 687-692

ISSN: 0910-6340

Publisher: Japan Society for Analytical Chemistry

Indexing

ISI Web of Knowledge - 5 Citations

ISI Web of Science

Scopus - 6 Citations

Scientific classification

CORDIS: Health sciences

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-003-K69

DOI: 10.2116/analsci.25.687

Resumo (PT): The present work describes a comparative study involving two sequential injection enzymatic procedures for the spectrophotometric determination of glucose in milk samples. The determinations were based on the use of the enzymes, glucose oxidase and peroxidase, in solution or immobilized, and 4-aminophenazone and phenol as chromogenic reagents. In the first procedure, 8.4 IU of glucose oxidase and 0.5 IU of peroxidase were consumed in each determination. In the second procedure, 107 IU of glucose oxidase and 105 IU of peroxidase, immobilized in glass beads, were sequentially packed into the same reactor. The reactor allowed us to perform about 600 continuous determinations with no activity loss. The proposed systems allowed the determination of glucose up to 120 mg L-1 in milk samples with a precision, considering the RSD (%) values, lower than 3.5%. The results obtained for the two systems were comparatively evaluated against a routine enzymatic method, showing a relative deviation of less than 3%. <br> <br> <a target="_blank" href="http://www.jstage.jst.go.jp/article/analsci/25/5/25_687/_article"> Texto integral</a> <br> <br>

Abstract (EN): The present work describes a comparative Study involving two Sequential injection enzymatic procedures for the spectrophotometric determination of glucose in milk Samples. The determinations were based oil the use of the enzymes, glucose oxidase and peroxidase, in solution or immobilized, and 4-aminophenazone and phenol as chromogenic reagents. In the first procedure, 8.4 IU of glucose oxidase and 0.5 IU of peroxidase were consumed in each determination. In the second procedure, 107 IU of glucose oxidase and 105 IU of peroxidase, immobilized in glass heads, Were sequentially packed into the same reactor. The reactor allowed LIS to perform about 600 continuous determinations with no activity loss. The proposed systems allowed the determination of glucose up to 120 mg L(-1) in milk samples with it precision, considering the RSD (%) values, lower than 3.5%. The results obtained for the two systems were Comparatively evaluated against it routine enzymatic method, showing it relative deviation of less than 3%.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6