Assessing the antioxidative properties and chemical composition of Linaria vulgaris infusion

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Vrchovska, V (Author) Other; Spilkova, J (Author) Other; Valentao, P (Author) FFUP; Sousa, C (Author) REIT; Andrade, PB (Author) FFUP; Seabra, RM (Author) Other

Journal

Title: Natural Product Research

Vol. 22 No. 17

Pages: 735-746

ISSN: 1478-6419

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 7 Citations

ISI Web of Science

Scopus - 11 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

CORDIS: Health sciences

Other information

Authenticus ID: P-004-476

DOI: 10.1080/14786410601132360

Resumo (PT): The ability of Linaria vulgaris (Scrophulariaceae) infusion to act as a scavenger of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, reactive oxygen species (superoxide radical, hydroxyl radical, hypochlorous acid (HOCl)) and nitric oxide was investigated. The obtained data indicate that the infusion has a good scavenging activity against superoxide radical and is a very potent nitric oxide and DPPH scavenger. In hydroxyl radical assay a pro-oxidant capacity was noticed, especially for concentrations higher than 31.25 µg mL-1. No effect was found against HOCl. A phytochemical study of this extract was also performed. The HPLC/UV analysis allowed the identification and quantification of eight organic acids (oxalic, aconitic, citric, ketoglutaric, ascorbic, malic, shikimic and fumaric acids). The phenolic composition of the lyophilised infusion was also determined by HPLC/DAD and four compounds were quantified, but, despite its high content, only linarin was managed to be identified. <br> <br> Keywords: Linaria vulgaris; DPPH; reactive oxygen species; nitric oxide; phenolic compounds; organic acids <br> <a target="_blank" href="http://www.informaworld.com/smpp/content~db=all~content=a793969747"> Texto integral</a> <br> <br>

Abstract (EN): The ability of Linaria vulgaris (Scrophulariaceae) infusion to act as a scavenger of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, reactive oxygen species (superoxide radical, hydroxyl radical, hypochlorous acid (HOCl)) and nitric oxide was investigated. The obtained data indicate that the infusion has a good scavenging activity against superoxide radical and is a very potent nitric oxide and DPPH scavenger. In hydroxyl radical assay a pro-oxidant capacity was noticed, especially for concentrations higher than 31.25 g mL(-1). No effect was found against HOCl. A phytochemical study of this extract was also performed. The HPLC/UV analysis allowed the identification and quantification of eight organic acids (oxalic, aconitic, citric, ketoglutaric, ascorbic, malic, shikimic and fumaric acids). The phenolic composition of the lyophilised infusion was also determined by HPLC/DAD and four compounds were quantified, but, despite its high content, only linarin was managed to be identified.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12