Changes in Red Blood Cells Membrane Protein Composition during Hemodialysis Procedure

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Elisio Costa (Author) FFUP; Susana Rocha (Author) FFUP; Petronila Rocha Pereira (Author) Other; Elisabeth Castro (Author) Other; Vasco Miranda (Author) Other; Maria do Sameiro Faria (Author) Other; Alfredo Loureiro (Author) Other; Alexandre Quintanilha (Author) ICBAS; Luis Belo (Author) FFUP; Alice Santos Silva (Author) FFUP

Journal

Title: Renal Failure

Vol. 30 No. 10

Pages: 971-975

ISSN: 0886-022X

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 12 Citations

ISI Web of Science

Scopus - 12 Citations

Scientific classification

FOS: Medical and Health sciences > Clinical medicine

CORDIS: Health sciences

Other information

Authenticus ID: P-004-4QJ

DOI: 10.1080/08860220802422036

Resumo (PT): Our aim was to evaluate the influence of the hemodialysis (HD) procedure in red blood cells (RBC) membrane protein composition. We evaluated hematological data (RBC count, hemoglobin concentration, and hematimetric indices) and RBC membrane protein composition (linear and exponential gradient polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate [SDS-PAGE] followed by densitometry analysis of RBC membrane proteins) before and immediately after the HD procedure in 20 patients (10 responders and 10 non-responders to recombinant human erythropoietin therapy [rhEPO]) and 26 healthy controls. Before HD, patients presented anaemia and significant changes in membrane protein composition, namely, a statistically significant reduction in spectrin associated with a significant increase in bands 6, as well as an altered membrane protein interaction (protein 4.1/spectrin, protein 4.1/band 3, protein 4.2/band 3 and spectrin/band 3). After HD, we found that patients showed a statistically significant increase in RBC count and hemoglobin, a further and statistically significant decrease in spectrin, an increase in band 3, and an altered spectrin/band 3 ratio. When comparing responders and non-responders patients after HD, we found that the non-responders presented a trend to a higher reduction in spectrin. Our data suggest that HD procedure seems to contribute to a reduction in spectrin, which is normally associated with a reduction in RBC deformability, being that reduction in spectrin is higher in non-responder patients <br> <br> Keywords: ERYTHROCYTE HEMODIALYSIS MEMBRANE proteins HEMATOLOGY DENSITOMETRY <br> <a target="_blank" href="http://web.ebscohost.com/ehost/detail?vid=3&hid=5&sid=c6a6a605-7f55-4daf-88cb-4fcf448e46d2%40SRCSM2&bdata=JnNpdGU9ZWhvc3QtbGl2ZSZzY29wZT1zaXRl#db=a9h&AN=35275905#db=a9h&AN=35275905#db=a9h&AN=35275905#db=a9h&AN=35275905#db=a9h&AN=35275905#db=a9h&AN=35275905#db=a9h&AN=35275905#db=a9h&AN=35275905#db=a9h&AN=35275905#db=a9h&AN=35275905"> Texto integral</a> <br> <br>

Abstract (EN): Our aim was to evaluate the influence of the hemodialysis (HD) procedure in red blood cells (RBC) membrane protein composition. We evaluated hematological data (RBC count, hemoglobin concentration, and hematimetric indices) and RBC membrane protein composition (linear and exponential gradient polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate [SDS-PAGE] followed by densitometry analysis of RBC membrane proteins) before and immediately after the HD procedure in 20 patients (10 responders and 10 non-responders to recombinant human erythropoietin therapy [rhEPO]) and 26 healthy controls. Before HD, patients presented anaemia and significant changes in membrane protein composition, namely, a statistically significant reduction in spectrin associated with a significant increase in bands 6, as well as an altered membrane protein interaction (protein 4.1/spectrin, protein 4.1/band 3, protein 4.2/band 3 and spectrin/band 3). After HD, we found that patients showed a statistically significant increase in RBC count and hemoglobin, a further and statistically significant decrease in spectrin, an increase in band 3, and an altered spectrin/band 3 ratio. When comparing responders and non-responders patients after HD, we found that the non-responders presented a trend to a higher reduction in spectrin. Our data suggest that HD procedure seems to contribute to a reduction in spectrin, which is normally associated with a reduction in RBC deformability, being that reduction in spectrin is higher in non-responder patients.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5