Sequential injection analysis as a tool for implementation of enzymatic assays in ionic liquids

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Paula C A G Pinto (Author) FFUP; Lucia L M F S Saraiva (Author) FFUP; Jose L F C Lima (Author) FFUP

Journal

Title: Talanta

Vol. 77 No. 2

Pages: 479-483

ISSN: 0039-9140

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 22 Citations

ISI Web of Science

Scopus - 23 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

CORDIS: Health sciences

Other information

Authenticus ID: P-003-T82

DOI: 10.1016/j.talanta.2008.03.017

Resumo (PT): An approach based on the use of water/1-butyl-3-methylimidazolium tetrafluoroborate ([bmim][BF4]) mixtures in a sequential injection analysis (SIA) system is presented. The rapid and robust procedure developed was used to evaluate horseradish peroxidase activity in [bmim][BF4] and is intended to be a generic tool for enzymatic assays in ionic liquids. The horseradish peroxidase activity tests were based on the implementation of the 4-aminoantipyrine (4AAP)/phenol test in the SIA system, using 1-naphtol as substrate. Small volumes (12 μL) of sample, reagents and enzyme were sequentially aspirated to the holding coil before being sent to the spectrophotometric detector (λ = 510 nm), where a coloured product proportional to the enzyme activity was measured. The results were compared to those obtained when the assay was performed in water/methanol mixtures under the same conditions, to evaluate [bmim][BF4] as an alternative to conventional organic solvents. Comparative evaluation of the enzyme behaviour revealed that the enzyme activity increased significantly when the assay was performed in water/[bmim][BF4] mixtures. The SIA methodology exhibited good repeatability over the full concentration range (R.S.D. < 3.3%, n = 15) studied, produced approximately 1.7 mL of effluent and consumed approximately 36 μL of solutions prepared in water/[bmim][BF4] mixtures for each analytical cycle. <br> <br> Keywords: SIA; Horseradish peroxidase; Ionic liquids; [Bmim][BF4]; Methanol <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6THP-4S4JYKY-3&_user=2460038&_coverDate=12%2F15%2F2008&_rdoc=6&_fmt=high&_orig=browse&_srch=doc-info(%23toc%235288%232008%23999229997%23700527%23FLA%23display%23Volume)&_cdi=5288&_sort=d&_docanchor=&_ct=76&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=8160fdde0916bc3a82a0cf042561ba8d"> Texto integral</a> <br> <br>

Abstract (EN): An approach based on the use of water/1-butyl-3-methylimidazolium tetrafluoroborate ([bmim][BF4]) mixtures in a sequential injection analysis (SIA) system is presented, The rapid and robust procedure developed was used to evaluate horseradish peroxidase activity in [bmim][BF4] and is intended to be a generic tool for enzymatic assays in ionic liquids. The horseradish peroxidase activity tests were based on the implementation of the 4-aminoantipyrine (4AAP)/phenol test in the SIA system, using 1-naphtol as substrate. Small Volumes (12 mu L) of sample, reagents and enzyme were sequentially aspirated to the holding coil before being sent to the spectrophotometric detector (lambda = 510 nm), where a Coloured product proportional to the enzyme activity was measured. The results were compared to those obtained when the assay was performed in water/methanol mixtures under the same conditions, to evaluate [bmim][BF4] as an alternative to conventional organic solvents. Comparative evaluation of the enzyme behaviour revealed that the enzyme activity increased significantly when the assay was performed in water/[bmim][BF4] mixtures. The SIA methodology exhibited good repeatability over the full concentration range(R.S.D. < 3.3%, n = 15) Studied, produced approximately 1.7mL of effluent and Consumed approximately 36 mu L of solutions prepared in water/[bmim][BF4] mixtures for each analytical cycle.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5