Alginate coated chitosan nanoparticles are an effective subcutaneous adjuvant for hepatitis B surface antigen

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Olga Borges (Author) Other; Marta Silva (Author) Other; Adriano de Sousa (Author) Other; Gerrit Borchard (Author) Other; Hans E Junginger (Author) Other; Anabela Cordeiro da Silva (Author) FFUP

Journal

Title: International Immunopharmacology

Vol. 8 No. 13

Pages: 1773-1780

ISSN: 1567-5769

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 59 Citations

ISI Web of Science

Scopus - 83 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

CORDIS: Health sciences

Other information

Authenticus ID: P-003-T6R

DOI: 10.1016/j.intimp.2008.08.013

Resumo (PT): We recently described a delivery system that is composed of a chitosan core to which the hepatitis B surface antigen (HBsAg) was adsorbed and subsequently coated with sodium alginate. In this present work, alginate coated chitosan nanoparticles were evaluated as a subcutaneous adjuvant for HBsAg. HBsAg loaded, alginate coated or uncoated chitosan nanoparticles, associated or not with CpGODN were subcutaneously administered to mice and several immunological parameters were evaluated. A high anti-HBsAg IgG titer (2271 ± 120 mIU/ml), with the majority of antibodies being of Th2 type, was observed within group I, vaccinated with HBsAg loaded onto coated nanoparticles. However, regarding cellular immune response, no significant differences were observed for antigen-specific splenocyte proliferation or for the secretion of IFN-γ and IL-4, when compared to the control group. The co-delivery of antigen-loaded nanoparticles in the presence of the immunopotentiator, CpG ODN 1826, resulted in an increase of anti-HBsAg IgG titers that was not statistically different from the first group; however, an increase of the IgG2a/IgG1 ratio from 0.1 to 1.0 and an increase (p < 0.01) of the IFN-γ production by the splenocytes stimulated with the HBV antigen was observed. The enhancement of the immune response observed with the antigen-loaded nanoparticles demonstrated that chitosan is a promising platform for parenteral HBsAg delivery and, when co-administered with the CpG ODN, resulted in a mixed Th1/Th2 type immune response. <br> <br> Keywords: Adjuvants; Subcutaneous vaccination; Hepatitis B surface antigen; CpG oligodeoxynucleotide; Alginate coated chitosan nanoparticles <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6W7N-4TFV6D6-1&_user=2460038&_coverDate=12%2F20%2F2008&_rdoc=11&_fmt=high&_orig=browse&_srch=doc-info(%23toc%236631%232008%23999919986%23700990%23FLA%23display%23Volume)&_cdi=6631&_sort=d&_docanchor=&_ct=28&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=f636edf9f307d94e82fe297474a77941"> Texto integral </a> <br> <br>

Abstract (EN): We recently described a delivery system that is composed of a chitosan core to which the hepatitis B surface antigen (HBsAg) was adsorbed and subsequently coated with sodium alginate. In this present work, alginate coated chitosan nanoparticles were evaluated as a subcutaneous adjuvant for HBsAg. HBsAg loaded, alginate coated or uncoated chitosan nanoparticles, associated or not with CpGODN were subcutaneously administered to mice and several immunological parameters were evaluated. A high anti-HBsAg IgG titer (2271 +/- 120 mIU/ml), with the majority of antibodies being of Th2 type, was observed within group 1, vaccinated with HBsAg loaded onto coated nanoparticles. However, regarding cellular immune response, no significant differences were observed for antigen-specific splenocyte proliferation or for the secretion of IFN-gamma and IL-4, when compared to the control group. The co-delivery of antigen-loaded nanoparticles in the presence of the immunopotentiator, CpG ODN 1826, resulted in an increase of anti-HBsAg IgG titers that was not statistically different from the first group; however, an increase of the IgG2a/IgG1 ratio from 0.1 to 1.0 and an increase (p<0.01) of the IFN-gamma production by the splenocytes stimulated with the HBV antigen was observed. The enhancement of the immune response observed with the antigen-loaded nanoparticles demonstrated that chitosan is a promising platform for parenteral HBsAg delivery and, when co-administered with the CpG ODN, resulted in a mixed Th1/Th2 type immune response.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8