Rapid microplate high-throughput methodology for assessment of Folin-Ciocalteu reducing capacity

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Luis M Magalhaes (Author) Other; Fernando Santos (Author) Other; Marcela A Segundo (Author) FFUP; Salette Reis (Author) FFUP; Jose L F C Lima (Author) FFUP

Journal

Title: Talanta

Vol. 83 No. 3

Pages: 441-447

ISSN: 0039-9140

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 55 Citations

ISI Web of Science

Scopus - 87 Citations

Pubmed / Medline

Scientific classification

FOS: Natural sciences > Chemical sciences

CORDIS: Health sciences > Pharmacological sciences > Pharmacy

Other information

Authenticus ID: P-003-038

DOI: 10.1016/j.talanta.2010.09.042

Resumo (PT): In the present work, a rapid and high-throughput Folin-Ciocalteu (F-C) reducing capacity assay adapted to routine/screening analysis was developed. In order to attain a fast F-C reducing kinetic reaction, the reaction conditions of the classical time-consuming F-C assay were modified and the influence of alkali and F-C reagent concentration was evaluated using gallic acid as standard. The proposed method was performed in a 96-well microplate format and it was applied to several phenolic compounds and food products (wines, beers, infusions and juices) providing F-C reducing capacity results after 3 min of reaction similar to those obtained by the time-consuming (120 min) conventional method. The additive and synergistic effect of reducing nonphenolic compounds usually found in food samples was also investigated. Ascorbic acid and ferrous sulfate provided an additive effect, while for fructose, glucose and sodium sulfite a synergistic effect was obtained. The detection limit was 0.25 mg L−1 (as gallic acid) and the repeatability was <1.6% (n = 12). <br> <br> Keywords: Folin-Ciocalteu; Microplate assay; Phenolic compounds; Food products <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6THP-514R64C-9&_user=2460038&_coverDate=12%2F15%2F2010&_rdoc=23&_fmt=high&_orig=browse&_origin=browse&_zone=rslt_list_item&_srch=doc-info(%23toc%235288%232010%23999169997%232693746%23FLA%23display%23Volume)&_cdi=5288&_sort=d&_docanchor=&_ct=60&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=d3ae9772ec07eda295e50c5d4d811c2f&searchtype=a"> Texto integral </a> <br>

Abstract (EN): In the present work, a rapid and high-throughput Folin-Ciocalteu (F-C) reducing capacity assay adapted to routine/screening analysis was developed. In order to attain a fast F-C reducing kinetic reaction, the reaction conditions of the classical time-consuming F-C assay were modified and the influence of alkali and F-C reagent concentration was evaluated using gallic acid as standard. The proposed method was performed in a 96-well microplate format and it was applied to several phenolic compounds and food products (wines, beers, infusions and juices) providing F-C reducing capacity results after 3 min of reaction similar to those obtained by the time-consuming (120 min) conventional method. The additive and synergistic effect of reducing nonphenolic compounds usually found in food samples was also investigated. Ascorbic acid and ferrous sulfate provided an additive effect, while for fructose, glucose and sodium sulfite a synergistic effect was obtained. The detection limit was 0.25 mg L(-1) (as gallic acid) and the repeatability was <1.6% (n = 12).

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7