Immunohistochemical Characterization of Adenosine Receptors in Rat Aorta and Tail Arteries

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Sandra Leal (Author) Other; Carlos, SA (Author) Other; Jorge Goncalves (Author) FFUP; Paula Fresco (Author) FFUP; Carmen Diniz (Author) FFUP

Journal

Title: Microscopy Research and Technique

Vol. 71 No. 10

Pages: 703-709

ISSN: 1059-910X

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 17 Citations

ISI Web of Science

Scopus - 18 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

CORDIS: Health sciences

Other information

Authenticus ID: P-003-VYX

DOI: 10.1002/jemt.20609

Resumo (PT): Adenosine plays an important role in the cardiovascular system, activating adenosine A1, A2A, A2B, and A3 receptors, and regulating blood flow either by acting directly on vascular cells or indirectly because of its effects on the central or peripheral nervous systems. The aim of the present study was to investigate whether the pattern of distribution of adenosine receptor subtypes is different on elastic and muscular, using abdominal aorta and tail arteries as models. Immunohistochemistry using anti-A1, anti-A2A, anti-A2B, and anti-A3 receptor antibodies was performed on perfused-fixed/paraffin-embedded arteries from Wistar rats. 3,3′-Diaminobenzidine tetrahydrochloride (DAB; activated by hydrogen peroxide) staining revealed significant differences in the abundance of A1, A2A, and A3 receptors between abdominal aorta and tail artery and allowed the identification of distinct distribution patterns for A1, A2A, A2B, and A3 receptors in the tunica adventitia, media, and intima of muscular and elastic arteries. Data are compatible with several previous functional reports supporting that different adenosine receptor subtype expression and/or their distribution in the vessel wall may influence their respective contribution to the control of blood flow <br> <br> <a target="_blank" href="http://onlinelibrary.wiley.com/doi/10.1002/jemt.20609/abstract">Texto integral</a> <br> <br>

Abstract (EN): Adenosine plays an important role in the cardiovascular system, activating adenosine A(1), A(2A), A(2)B, and A(3) receptors, and regulating blood flow either by acting directly on vascular cells or indirectly because of its effects on the central or peripheral nervous systems. The aim of the present study was to investigate whether the pattern of distribution of adenosine receptor subtypes is different on elastic and muscular, using abdominal aorta and tail arteries as models. Immunohistochemistry using anti-A(1), anti-A(2A), anti-A(2B), and anti-A(3) receptor antibodies was performed on perfused-fixed/paraffin-embedded arteries from Wistar rats. 3,3'-Diaminobenzidine tetrahydrochloride (DAB; activated by hydrogen peroxide) staining revealed significant differences in the abundance of A,, A2A, and A3 receptors between abdominal aorta and tail artery and allowed the identification of distinct distribution patterns for A(1), A(2A), A(2B), and A(3) receptors in the tunica adventitia, media, and intima of muscular and elastic arteries. Data are compatible with several previous functional reports supporting that different adenosine receptor subtype expression and/or their distribution in the vessel wall may influence their respective contribution to the control of blood flow. Microsc. Res. Tech. 71:703-709, 2008. (C) 2008 Wiley-Liss, Inc.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7