Effects of resveratrol on membrane biophysical properties: relevance for its pharmacological effects

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Brittes, J (Author) Other; Lucio, M (Author) FEUP; Nunes, C (Author) Other; Lima, JLFC (Author) FFUP; Reis, S (Author) FFUP

Journal

Title: Chemistry and Physics of Lipids

Vol. 163 No. 8

Pages: 747-754

ISSN: 0009-3084

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 72 Citations

ISI Web of Science

Scopus - 81 Citations

Pubmed / Medline

Scientific classification

CORDIS: Health sciences > Pharmacological sciences > Pharmacy

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-003-191

DOI: 10.1016/j.chemphyslip.2010.07.004

Resumo (PT): The current study gathers a range of spectrophotometric and spectrofluorimetric techniques to systematically monitor the effects of resveratrol (trans-3,5,4′-trihydrostilbene) on the biophysical properties of membrane model systems consisting of unilamellar liposomes of phosphatidylcholine (DPPC) with the ultimate goal of relating these effects with some of the well documented pharmacological properties of this compound, and clarifying some controversial results reported on the literature. Physiological conditions have been pursued, such as a buffered pH control with adjusted ionic strength similar to the blood plasma conditions (pH 7.4, I = 0.1 M) and the study at different membrane physical states (gel phase and fluid phase) for the assessment of resveratrol-membrane: aqueous partition coefficient by derivative spectroscopy. Results obtained by fluorescence quenching and anisotropy studies indicate that resveratrol has a membrane fluidizing effect and is able to permeate the membrane even in the gel phase. These results mirror the well described antioxidant effect of resveratrol, since antioxidants have to reach peroxidised rigid membranes and increase membrane fluidity in order to interact more efficiently with lipid radicals in the disordered lipid bilayer. Location of resveratrol pointed also to a membrane distribution that is favourable for scavenging the lipid radicals and was elucidated using probes positioned at different membrane depths suggesting that this compound penetrates into the acyl membrane region but also positions its polar hydroxyl group near the headgroup region of the membrane. <br> <br> Keywords: Resveratrol; Liposomes; Fluorescence quenching; Steady-state anisotropy; Derivative spectrophotometry <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T2N-50PCMDR-1&_user=2460038&_coverDate=11%2F30%2F2010&_rdoc=2&_fmt=high&_orig=browse&_origin=browse&_zone=rslt_list_item&_srch=doc-info(%23toc%234923%232010%23998369991%232564739%23FLA%23display%23Volume)&_cdi=4923&_sort=d&_docanchor=&_ct=10&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=cacc4863694258b3d638047aadf60fe2&searchtype=a "> Texto integral</a> <br> <br>

Abstract (EN): The current study gathers a range of spectrophotometric and spectrofluorimetric techniques to systematically monitor the effects of resveratrol (trans-3,5,4'-trihydrostilbene) on the biophysical properties of membrane model systems consisting of unilamellar liposomes of phosphatidylcholine (DPPC) with the ultimate goal of relating these effects with some of the well documented pharmacological properties of this compound, and clarifying some controversial results reported on the literature. Physiological conditions have been pursued, such as a buffered pH control with adjusted ionic strength similar to the blood plasma conditions (pH 7.4. I=0.1 M) and the study at different membrane physical states (gel phase and fluid phase) for the assessment of resveratrol-membrane: aqueous partition coefficient by derivative spectroscopy. Results obtained by fluorescence quenching and anisotropy studies indicate that resveratrol has a membrane fluidizing effect and is able to permeate the membrane even in the gel phase. These results mirror the well described antioxidant effect of resveratrol, since antioxidants have to reach peroxidised rigid membranes and increase membrane fluidity in order to interact more efficiently with lipid radicals in the disordered lipid bilayer. Location of resveratrol pointed also to a membrane distribution that is favourable for scavenging the lipid radicals and was elucidated using probes positioned at different membrane depths suggesting that this compound penetrates into the acyl membrane region but also positions its polar hydroxyl group near the headgroup region of the membrane.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8