Indirect Sequential Injection Enzymatic Determination of Allopurinol in Pharmaceuticals Based on Xanthine Oxidase Inhibition

• Type: Article in International Scientific Journal
• Date: 2009

Authors

Cristina I C Silvestre (Author) Other; Marcela A Segundo (Author) FFUP; Lucia L M F S Saraiva (Author) FFUP; Jose L F C Lima (Author) FFUP

Journal

Title: Spectroscopy Letters

Vol. 42 No. 6

Pages: 341-350

ISSN: 0038-7010

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 2 Citations

ISI Web of Science

Scopus - 3 Citations

Scientific classification

FOS: Natural sciences > Physical sciences

CORDIS: Health sciences

Other information

Authenticus ID: P-003-S8F

DOI: 10.1080/00387010903185538

Resumo (PT): Two automatic sequential injection analysis methodologies were developed for the enzymatic determination of allopurinol in pharmaceutical formulations. Both methodologies were based on the inhibition by allopurinol of the reaction catalysed by xanthine oxidase, assessed by spectrophotometric detection of the reaction product (uric acid) at 295 nm. One methodology was established after a single readout at a fixed time while the other was based on the decrease of the rate of formation of uric acid upon monitoring the absorbance change. The analytical performance of both methodologies was validated, providing low environmental impact tools for assessment of allopurinol in drugs. <br> <br> Keywords: allopurinol; automation; enzymatic determination; Green Analytical Chemistry; sequential injection analysis; xanthine oxidase inhibitor <br> <a target="_blank" href="http://www.informaworld.com/smpp/content~db=all~content=a917315030~frm=titlelink "> Texto integral </a> <br> <br>

Abstract (EN): Two automatic sequential injection analysis methodologies were developed for the enzymatic determination of allopurinol in pharmaceutical formulations. Both methodologies were based on the inhibition by allopurinol of the reaction catalysed by xanthine oxidase, assessed by spectrophotometric detection of the reaction product (uric acid) at 295nm. One methodology was established after a single readout at a fixed time while the other was based on the decrease of the rate of formation of uric acid upon monitoring the absorbance change. The analytical performance of both methodologies was validated, providing low environmental impact tools for assessment of allopurinol in drugs.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10