Rapid chemiluminometric determination of gabapentin in pharmaceutical formulations exploiting pulsed-flow analysis

• Type: Article in International Scientific Journal
• Date: 2009

Authors

Matias Manera (Author) Other; Manuel Miro (Author) Other; Marta F T Ribeiro (Author) Other; Jose M Manuel Estelaa (Author) Other; Victor Cerda (Author) Other; Joao L M Santos (Author) FFUP; Jose L F C Lima (Author) FFUP

Journal

Title: Luminescence

Vol. 24

Pages: 10-14

ISSN: 1522-7235

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 14 Citations

ISI Web of Science

Scopus - 20 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

CORDIS: Health sciences

Other information

Authenticus ID: P-003-R7J

DOI: 10.1002/bio.1055

Resumo (PT): In this study, a straightforward and automated pulsed flow-based procedure was developed for the chemiluminometric determination of gabapentin [1-(aminomethyl)cyclo-hexaneacetic acid], a new generation antiepileptic drug, in different formulated dosage forms. The software-controlled time-based injection method capitalizes on the decrease of the background chemiluminescence (CL) readout of the luminol-hypochlorite reaction in the presence of gabapentin. In short, gabapentin works as a hypochlorite scavenger. The analytical procedure was implemented in a multi-pumping flow network furnished with a suite of microdispensing solenoid-actuated pumps. The diaphragm-type micropumps might be configured to operate as fluid propellers, commutation units and metering injectors. A dynamic linear working range for gabapentin concentrations in the range 60-350 µmol/L was obtained, with an estimated detection limit of 40 µmol/L. The flow analyser handles about 41 injections/h and yields precise results (RSD < 2%). The miniaturized flow analyser thus has potential to be exploited for in-line monitoring of drug manufacturing within the quality assurance framework of modern pharmaceutical companies <br> <br> Keywords gabapentin • chemiluminescence • pulsed-flow analysis • pharmaceutical assays <br> <a target="_blank" href="http://www3.interscience.wiley.com/journal/121401919/abstract"> Texto integral</a> <br> <br>

Abstract (EN): In this study, a straightforward and automated pulsed flow-based procedure was developed for the chemiluminometric determination of gabapentin [1-(aminomethyl)cyclo-hexaneacetic acid], a new generation antiepileptic drug, in different formulated dosage forms. The software-controlled time-based injection method capitalizes on the decrease of the background chemiluminescence (CL) readout of the luminol-hypochlorite reaction in the presence of gabapentin. In short, gabapentin works as a hypochlorite scavenger. The analytical procedure was implemented in a multi-pumping flow network furnished with a suite of microdispensing solenoid-actuated pumps. The diaphragm-type micropumps might be configured to operate as fluid propellers, commutation units and metering injectors. A dynamic linear working range for gabapentin concentrations in the range 60-350 gmol/L was obtained, with an estimated detection limit of 40 gmol/L. The flow analyser handles about 41 injections/h and yields precise results (RSD < 2%). The miniaturized flow analyser thus has potential to be exploited for in-line monitoring of drug manufacturing within the quality assurance framework of modern pharmaceutical companies. Copyright (c) 2008 John Wiley & Sons, Ltd.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5