In Vitro Cultures of Brassica oleracea L. var. costata DC: Potential Plant Bioreactor for Antioxidant Phenolic Compounds

• Type: Article in International Scientific Journal
• Date: 2009

Authors

Marcos Taveira (Author) Other; David M Pereira (Author) Other; Carla Sousa (Author) FFUP; Federico Ferreres (Author) Other; Paula B Andrade (Author) FFUP; Anabela Martins (Author) Other; Jose A Pereira (Author) Other; Patricia Valentao (Author) FFUP

Journal

Title: Journal of Agricultural and Food Chemistry

Vol. 57 No. 19

Pages: 1247-1252

ISSN: 0021-8561

Publisher: American Chemical Society

Indexing

ISI Web of Knowledge - 20 Citations

Scopus - 28 Citations

Scientific classification

FOS: Agrarian Sciences > Other Agrarian Sciences

Other information

Authenticus ID: P-003-N4H

DOI: 10.1021/jf803496x

Resumo (PT): In this work were studied the phenolic composition of in vitro material (shoots, calli, and roots) of Brassica oleracea var. costata and its antioxidant capacity. Samples were obtained in different culture medium, with distinct supplementations to verify their influence on those parameters. Phenolic determination was achieved by HPLC-DAD. Antioxidant activity was assessed against DPPH•. In calli and roots no phenolic compound was identified. In shoots was verified the presence of 36 compounds, which included hydroxycinnamic acids, flavonoids (kaempferol and quercetin derivatives), and hydroxycinnamic acyl glycosides (with a predominance of synapoyl gentiobiosides). MS liquid medium supplemented with 2 mg/L benzylaminopurine (BAP) and 0.1 mg/L naphthaleneacetic acid (NAA) revealed to be the best in vitro condition to produce shoot material with highest phenolic compound contents and stronger antioxidant potential, thus with a possible increase of health benefits. <br> <br> <a target="_blank" href="http://pubs.acs.org/doi/abs/10.1021/jf803496x"> Texto integral </a> <br> <br>

Abstract (EN): In this work were studied the phenolic composition of in vitro material (shoots, calli, and roots) of Brassica oleracea var. costata and its antioxidant capacity. Samples were obtained in different culture medium, with distinct supplementations to verify their influence on those parameters. Phenolic determination was achieved by HPLC-DAD. Antioxidant activity was assessed against DPPH(center dot). In calli and roots no phenolic compound was identified. In shoots was verified the presence of 36 compounds, which included hydroxycinnamic acids, flavonoids (kaempferol and quercetin derivatives), and hydroxycinnamic acyl glycosides (with a predominance of synapoyl gentiobiosides). MS liquid medium supplemented with 2 mg/L benzylaminopurine, (BAP) and 0.1 mg/L naphthaleneacetic acid (NAA) revealed to be the best in vitro condition to produce shoot material with highest phenolic compound contents and stronger antioxidant potential, thus with a possible increase of health benefits.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6