High diversity of extended-spectrum beta-lactamases among clinical isolates of Enterobacteriaceae from Portugal

• Type: Article in International Scientific Journal
• Date: 2007

Authors

Elisabete Machado (Author) Other; Teresa M Coque (Author) Other; Rafael Canton (Author) Other; Angela Novais (Author) Other; Joao Carlos Sousa (Author) Other; Fernando Baquero (Author) Other; Luisa Peixe (Author) FFUP

Journal

Title: Journal of Antimicrobial Chemotherapy

Vol. 60 No. 6

Pages: 1370-1374

ISSN: 0305-7453

Publisher: Oxford University Press

Indexing

ISI Web of Knowledge - 52 Citations

Scopus - 59 Citations

Pubmed / Medline

Scientific classification

CORDIS: Health sciences

FOS: Medical and Health sciences > Health sciences

Other information

Authenticus ID: P-004-61C

DOI: 10.1093/jac/dkm381

Resumo (PT): To investigate the occurrence and the diversity of Ambler class A ESBLs among Enterobacteriaceae from different Portuguese clinical settings over a 2 year period (2002–04). Methods One hundred and nine extended-spectrum ß-lactamase (ESBL)-producing Enterobacteriaceae isolates from five geographically distant health institutions in Portugal were studied. ESBLs were characterized by isoelectric focussing, PCR and further sequencing. Antibiotic susceptibility testing, transfer of resistance genes and clonal diversity were determined by standard procedures. Plasmid relatedness was established by comparison of random amplified polymorphic DNA (RAPD) patterns. Results ESBLs were identified as TEM (46%), SHV (30%), CTX-M (22%) and GES (2%) types; TEM-24, TEM-52, SHV-12 and CTX-M-15 enzymes being the most frequently found. Inter-hospital dissemination of epidemic strains harbouring the most prevalent ESBLs was detected, including the TEM-24-producing Enterobacter aerogenes European epidemic clone. Conjugative transfer of ESBLs was achieved for 67% of isolates and epidemic plasmids containing specific bla genes were detected (blaCTX-M-15 and blaTEM-24). We describe two new ESBLs, SHV-90 (A187T, G238S and E240K) and SHV-91 (P20S and E240K), and a new TEM-type enzyme conferring a phenotype resembling that of a complex mutant TEM ß-lactamase, designated as TEM-154 (M69L and R164S). The broad-spectrum ß-lactamases SHV-26, SHV-36 and TEM-110 were first observed in our country. Conclusions We describe a complex ESBL epidemiology in Portugal, including widespread dissemination of known strains and plasmids coding for TEM-24 and CTX-M-15 enzymes as observed in other European countries. <br> <br> <a target="_blank" href="http://ejournals.ebsco.com/Article.asp?ContributionID=13148454">Texto integral </a> <br> <br>

Abstract (EN): Objectives: To investigate the occurrence and the diversity of Ambler class A ESBLs among Enterobacteriaceae from different Portuguese clinical settings over a 2 year period (2002-04). Methods: One hundred and nine extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae isolates from five geographically distant health institutions in Portugal were studied. ESBLs were characterized by isoelectric focussing, PCR and further sequencing. Antibiotic susceptibility testing, transfer of resistance genes and clonal diversity were determined by standard procedures. Plasmid relatedness was established by comparison of random amplified polymorphic DNA (RAPD) patterns. Results: ESBLs were identified as TEM (46%), SHV (30%), CTX-M (22%) and GES (2%) types; TEM-24, TEM-52, SHV-12 and CTX-M-15 enzymes being the most frequently found. Inter-hospital dissemination of epidemic strains harbouring the most prevalent ESBLs was detected, including the TEM-24-producing Enterobacter aerogenes European epidemic clone. Conjugative transfer of ESBLs was achieved for 67% of isolates and epidemic plasmids containing specific bla genes were detected (bla(CTX-M-15) and bla(TEM-24)). We describe two new ESBLs, SHV-90 (A187T, G238S and E240K) and SHV-91 (P20S and E240K), and a new TEM-type enzyme conferring a phenotype resembling that of a complex mutant TEM beta-lactamase, designated as TEM-154 (M69L and R164S). The broad-spectrum beta-lactamases SHV-26, SHV-36 and TEM-110 were first observed in our country. Conclusions: We describe a complex ESBL epidemiology in Portugal, including widespread dissemination of known strains and plasmids coding for TEM-24 and CTX-M-15 enzymes as observed in other European countries.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5