A Chemiluminescence Flow-based Procedure for Determination of Carbaryl in Natural Waters Exploiting Multicommutation and Enzymatic Reaction

• Type: Article in International Scientific Journal
• Date: 2007

Authors

Cherrine K. Pires (Author) Other; Angel Morales-Rubio (Author) Other; Miguel de la Guardia (Author) Other; José L. F. C. Lima (Author) FFUP; Elias A. G. Zagatto (Author) Other; Boaventura F. Reis (Author) Other

Journal

Title: Journal of the Brazilian Chemical Society

Vol. 18 No. 3

Pages: 519-525

ISSN: 0103-5053

Publisher: Sociedade Brasileira de Quimica

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 5 Citations

Scientific classification

FOS: Medical and Health sciences > Other medical sciences

CORDIS: Health sciences

Other information

Authenticus ID: P-004-DRW

Resumo (PT): Um procedimento empregando detecção quiluminescente para determinação de carbaril em águas naturais utilizando acetilcolinesterase e colina oxidase é descrito. O módulo de análise projetado para implementar o processo de multicomutação controlado por microcomputador foi constituído por cinco válvulas solenóides, duas colunas com enzimas imobilizadas em esferas de vidro e cela de fluxo para detecção de quimiluminescência. Nas melhores condições experimentais foi obtida faixa linear entre 25 a 700 μg L-1 de carbaril. Amostras de água foram fortificadas com carbaril e as recuperações variaram entre 95 e 101%. Limite de detecção e coeficiente de variação foram estimados em 11 μg L-1 (99,7% nível de confiança) e 1,3% (n = 20), respectivamente. Outras características analíticas tais como freqüência de amostragem de 72 determinações por hora, consumo de reagente de 2,1 mg de hexacianoferrato de potássio(III) e 0,069 mg de luminol por determinação, também, foram obtidas. <br> <br> Keywords: multicommutation, flow analysis, pesticide carbaryl, chemiluminescence, enzymatic reaction <br> <a target="_blank" href="http://jbcs.sbq.org.br/jbcs/2007/vol18_n3/index.htm"> Texto integral</a> <br> <br>

Abstract (EN): A chemiluminescence procedure for the determination of carbaryl in natural waters using acetylcholinesterase and choline oxidase is described. The flow system designed to implement multicommutation approach controlled by microcomputer comprised five solenoid valves, two columns with immobilized enzymes on controlled pore glass beads and chemiluminometric flow cell. In the best experimental conditions a linear response ranging from 25 to 700 μg L-1 carbaryl was obtained. Water samples were spiked with carbaryl in order to access the accuracy and recoveries between 95 and 101% were obtained for a concentration level ranging from 25 to 100 μg L-1 carbaryl. Detection limit and variation coefficient were estimated as 11 μg L-1 (99.7% confidence level) and 1.3% (n = 20), respectively. Other profitable features such as a sample throughput of 72 determinations per hour, a reagent consumption of 2.1 mg potassium hexacyano ferrate(III) and 0.069 mg luminol per determination were also achieved. <br> <br> Keywords: multicommutation, flow analysis, pesticide carbaryl, chemiluminescence, enzymatic reaction <br> <a target="_blank" href="http://jbcs.sbq.org.br/jbcs/2007/vol18_n3/index.htm"> Full text </a> <br> <br>

Language: Portuguese

Type (Professor's evaluation): Scientific