Anti-Leishmania humoral and cellular immune responses in naturally infected symptomatic and asymptomatic dogs

• Type: Article in International Scientific Journal
• Date: 2007

Authors

L. Cardoso (Author) Other; H.D.F.H. Schallig (Author) Other; A. Cordeiro-da-Silva (Author) FFUP; M. Cabral (Author) FFUP; J.M. Alunda (Author) Other; M. Rodrigues (Author) Other

Journal

Title: Veterinary Immunology and Immunopathology

Vol. 117 No. 1

Pages: 35-41

ISSN: 0165-2427

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 30 Citations

ISI Web of Science

Scopus - 36 Citations

Scientific classification

FOS: Medical and Health sciences > Other medical sciences

Other information

Authenticus ID: P-004-A42

Resumo (PT): Canine infections with Leishmania infantum represent a considerable veterinary medical and public health problem. In this study, immunoglobulin G1 (IgG1) and IgG2 specific humoral responses were measured and compared with the delayed type hypersensitivity (DTH) cellular response to a leishmanin, in three groups of dogs clinically and serologically characterised as: (I) asymptomatic and direct agglutination test (DAT)-seronegative; (II) asymptomatic and DAT-seropositive; (III) DAT-seropositive and symptomatic. IgG2 was regarded as a marker of disease, since significantly higher levels of this subclass were recorded in the symptomatic dogs. In contrast, the IgG1 response could not be related to clinically relevant infection. A high correlation was observed between IgG2 level and DAT titre; the correlations between IgG1 and IgG2 levels, and between IgG1 level and DAT titre were lower. This may indicate that IgG2 is the main subclass in the specific humoral response which is detected by the DAT. A reduced IgG2 response, albeit not significantly different, was recorded among dogs with clear cellular immune responses detected by a DTH positive reaction. Furthermore, no correlations were observed between cellular response measured by DTH and humoral responses quantified by DAT titre or IgG1 and IgG2 levels. Combining serology and DTH skin test is a practical procedure to assess anti-Leishmania immune responses in dogs. <br> <br> Keywords: Direct agglutination test; Dog; IgG1; IgG2; Leishmania infantum; Leishmanin skin test <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TD5-4MY6G7V-7&_user=2460038&_coverDate=05%2F15%2F2007&_rdoc=5&_fmt=high&_orig=browse&_srch=doc-info(%23toc%235189%232007%23998829998%23649644%23FLA%23display%23Volume)&_cdi=5189&_sort=d&_docanchor=&_ct=18&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=04f6f358946db7c20b686f215d8e29b2"> Texto integral</a> <br> <br>

Abstract (EN): Canine infections with Leishmania infantum represent a considerable veterinary medical and public health problem. In this study, immunoglobulin G1 (IgG1) and IgG2 specific humoral responses were measured and compared with the delayed type hypersensitivity (DTH) cellular response to a leishmanin, in three groups of dogs clinically and serologically characterised as: (I) asymptomatic and direct agglutination test (DAT)-seronegative; (II) asymptomatic and DAT-seropositive; (III) DAT-seropositive and symptomatic. IgG2 was regarded as a marker of disease, since significantly higher levels of this subclass were recorded in the symptomatic dogs. In contrast, the IgG1 response could not be related to clinically relevant infection. A high correlation was observed between IgG2 level and DAT titre; the correlations between IgG1 and IgG2 levels, and between IgG1 level and DAT titre were lower. This may indicate that IgG2 is the main subclass in the specific humoral response which is detected by the DAT. A reduced IgG2 response, albeit not significantly different, was recorded among dogs with clear cellular immune responses detected by a DTH positive reaction. Furthermore, no correlations were observed between cellular response measured by DTH and humoral responses quantified by DAT titre or IgG1 and IgG2 levels. Combining serology and DTH skin test is a practical procedure to assess anti-Leishmania immune responses in dogs. <br> <br> Keywords: Direct agglutination test; Dog; IgG1; IgG2; Leishmania infantum; Leishmanin skin test <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TD5-4MY6G7V-7&_user=2460038&_coverDate=05%2F15%2F2007&_rdoc=5&_fmt=high&_orig=browse&_srch=doc-info(%23toc%235189%232007%23998829998%23649644%23FLA%23display%23Volume)&_cdi=5189&_sort=d&_docanchor=&_ct=18&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=04f6f358946db7c20b686f215d8e29b2"> Full text</a> <br> <br>

Language: Portuguese

Type (Professor's evaluation): Scientific