Comprehensive Metabolomics and Lipidomics Profiling of Prostate Cancer Tissue Reveals Metabolic Dysregulations Associated with Disease Development

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Lima, AR (Author) Other; Carvalho, M (Author) Other; Aveiro, SS (Author) Other; Melo, T (Author) Other; Domingues, MR (Author) Other; Macedo-Silva, C (Author) Other; Coimbra, N (Author) Other; Carmen Jeronimo (Author) ICBAS; henrique, rmf (Author) ICBAS; Maria de Lourdes Bastos (Author) FFUP; de Pinho, PG (Author) FCUP; Pinto, J (Author) Other

Journal

Title: Journal of Proteome Research

Vol. 21

Pages: 727-739

ISSN: 1535-3893

Publisher: American Chemical Society

Indexing

ISI Web of Knowledge - 9 Citations

Scopus - 10 Citations

Other information

Authenticus ID: P-00V-RZQ

DOI: 10.1021/acs.jproteome.1c00754

Abstract (EN): Prostate cancer (PCa) is a global health problem that affects millions of men every year. In the past decade, metabolomics and related subareas, such as lipidomics, have demonstrated an enormous potential to identify novel mechanisms underlying PCa development and progression, providing a good basis for the development of new and more effective therapies and diagnostics. In this study, a multiplatform metabolomics and lipidomics approach, combining untargeted mass spectrometry (MS) and nuclear magnetic resonance (NMR)-based techniques, was applied to PCa tissues to investigate dysregulations associated with PCa development, in a cohort of 40 patients submitted to radical prostatectomy for PCa. Results revealed significant alterations in the levels of 26 metabolites and 21 phospholipid species in PCa tissue compared with adjacent nonmalignant tissue, suggesting dysregulation in 13 metabolic pathways associated with PCa development. The most affected metabolic pathways were amino acid metabolism, nicotinate and nicotinamide metabolism, purine metabolism, and glycerophospholipid metabolism. A clear interconnection between metabolites and phospholipid species participating in these pathways was observed through correlation analysis. Overall, these dysregulations may reflect the reprogramming of metabolic responses to produce high levels of cellular building blocks required for rapid PCa cell proliferation.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13